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SM067RT CD161 / KLRB1 antibody

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Mouse anti Mouse CD161 / KLRB1 PK136

SM067RT

Product Description for CD161 / KLRB1

Mouse anti Mouse CD161 / KLRB1 PK136.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for CD161 / KLRB1

Product Category Primary Antibodies
Quantity 25 Tests
Synonyms C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A
Presentation PE
Reactivity Ms
Applications F
Clonality Monoclonal
Clone PK136
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q12918
Immunogen:
Spleen and bone marrow cells from CE mice
GeneID:
3820
Application Flow cytometry: use 10 ul of neat antibody to label 10e6 cells in 100 µl.
Background NK1.1 cell surface antigen is a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.
Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.
General Readings
  1. Koo GC, Peppard JR. Establishment of monoclonal anti-Nk-1.1 antibody. Hybridoma. 1984 Fall;3(3):301-3. PubMed PMID: 6500587.
  2. Koo GC, Dumont FJ, Tutt M, Hackett J, Kumar V. The NK-1.1(-) mouse: a model to study differentiation of murine NK cells. J Immunol. 1986 Dec 15;137(12):3742-7. PubMed PMID: 3782794.
  3. Kung, S.K.P. and Miller, R.G. (1995) The NK1.1 antigen in NK-mediated FI antiparent killing in vitro. J. Immunol. 154: 1624-1633.
  4. Wang M, Ellison CA, Gartner JG, HayGlass KT. Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses. J Immunol. 1998 Feb 1;160(3):1098-105. PubMed PMID: 9570522.
  5. Arase N, Arase H, Park SY, Ohno H, Ra C, Saito T. Association with FcRgamma is essential for activation signal through NKR-P1 (CD161) in natural killer (NK) cells and NK1.1+ T cells. J Exp Med. 1997 Dec 15;186(12):1957-63. PubMed PMID: 9396764. (Free PMC Article available, 7 images available)
  6. Carlyle JR, Martin A, Mehra A, Attisano L, Tsui FW, Zúñiga-Pflücker JC. Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function. J Immunol. 1999 May 15;162(10):5917-23. PubMed PMID: 10229828.
Storage Prior to and following reconstitution store undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R. Phycoerythrin (RPE)
PE
Purification:
Affinity Chromatography on Protein A.
Buffer System:
PBS, pH 7.4, containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with distilled water.
Specificity
Specificity:
The antibody recognizes the mouse NK1.1 cell surface antigen.
This antibody is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c).
The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
Species:
Mouse. Does not react with Rat and Human.
Gene ID 3820

Accessory Products

Proteins and/or Positive Controls

Proteins for CD161 / KLRB1 (5 products)

Catalog No. Species Pres. Purity   Source  

CD161 / KLRB1 (67-225, His-tag)
CD161 / KLRB1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

CD161 / KLRB1 (67-225, His-tag)
CD161 / KLRB1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

CD161 / KLRB1
CD161 / KLRB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD161 / KLRB1
CD161 / KLRB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD161 / KLRB1
CD161 / KLRB1 Human
  Abnova Taiwan Corp.

Positive controls for CD161 / KLRB1 (1 products)

Catalog No. Species Pres. Purity   Source  

KLRB1 overexpression lysate
KLRB1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

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Click here to see all secondary antibodies for CD161 / KLRB1
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