Product Description for PRAME / MAPE
Rabbit anti Human PRAME / MAPE.
Properties: (C-term)
Product is tested for Paraffin Sections, Flow Cytometry, Western blot / Immunoblot.
Properties: (C-term)
Product is tested for Paraffin Sections, Flow Cytometry, Western blot / Immunoblot.
Properties for PRAME / MAPE
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.4 ml |
| Synonyms | Melanoma antigen preferentially expressed in tumors, OIP-4, OIP4, OPA-interacting protein 4, Preferentially expressed antigen of melanoma |
| Reactivity | Hu |
| Applications | F, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | C-term |
| Application | Peptide ELISA: 1/1,000. Flow Cytometry: 1/25. Western blotting: 1/2,000. Immunohistochemistry on Paraffin Sections: 1/50-1/100. |
| Background | MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias. |
| Concentration | 0.5 mg/ml (lot specific) |
| General Readings |
|
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
|
| Specificity |
|
| Gene ID | 23532 |
Accessory Products
Proteins and/or Positive Controls
Proteins for PRAME / MAPE (6 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP307874 |
PRAME / MAPE (transcript variant 4) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP760464 |
PRAME / MAPE (N-term HIS tag, transcript variant 3) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP760913 |
PRAME / MAPE (full length, N-term HIS tag, transcript variant 5) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR60005PU-N |
PRAME / MAPE |
||||||
|
Human | Purified | > 98 % by SDS-PAGE and Silver staining | E. coli |
20 µg /
€200.00
|
||
| OriGene Technologies GmbH | |||||||
| H00023532-P01-10 |
PRAME / MAPE |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00023532-P01-25 |
PRAME / MAPE |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for PRAME / MAPE (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00023532-T01 |
PRAME 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| NBL1-14723 |
PRAME Lysate |
||||||
![Western Blot: PRAME Lysate [NBL1-14723] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PRAME.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/PRAME%20Lysate-Western%20Blot-NBL1-14723-img0001.jpg) |
|||||||
| Novus Biologicals Inc. | |||||||
| NBL1-14724 |
PRAME Lysate |
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![Western Blot: PRAME Lysate [NBL1-14724] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for PRAME](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/PRAME%20Lysate-Western%20Blot-NBL1-14724-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
| NBL1-14725 |
PRAME Lysate |
||||||
![Western Blot: PRAME Lysate [NBL1-14725] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for PRAME](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/PRAME%20Lysate-Western%20Blot-NBL1-14725-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for PRAME / MAPE (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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