Product Description for Peroxiredoxin-6 / PRDX6
Peroxiredoxin-6 / PRDX6.
Presentation: Purified
Presentation: Purified
Properties for Peroxiredoxin-6 / PRDX6
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | 1-Cys PRX, 1-Cys peroxiredoxin, AOP-2, AOP2, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, KIAA0106, Liver 2D page spot 40, NSGPx, Non-selenium glutathione peroxidase, PRDX-6, Prx-6, Prx6, Red blood cells page spot 12, aiPLA2 |
| Presentation | Purified |
| Molecular weight | 27.1 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
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| Purity | > 95 % by SDS - PAGE |
| Background | Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay: 1.Prepare a 50ul reaction mix into a suitable container : The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2.Add 5 ul of recombinant PRDX6 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3.Incubate at 25°C for 2 minutes. 4.Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5.Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6.Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) 7.Record the increase in A475nm. |
| General Readings | Davison EJ., et al. (2009). Proteomics. 9(18):4284-97. Wu J., et al. (2009). Chem Res Toxicol. 22(4):668-75. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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