Product Description for FKBPL
FKBPL.
Presentation: Purified
Presentation: Purified
Properties for FKBPL
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 50 µg |
| Synonyms | DIR1, FK506-binding protein-like, NG7, WAF-1/CIP1 stabilizing protein 39, WISp39 |
| Presentation | Purified |
| Molecular weight | 39.2 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 90 % by SDS - PAGE |
| Background | FKBPL has similarity to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is thought to have a potential role in the induced radioresistance and it appears to have some involvement in the control of the cell cycle. Also, FKBPL is involved in cellular response to stress. And it is known to interact with Hsp90, glucocorticoid receptor and dynamitin and may play a role in signalling, like other FKBPs. |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2.Add 10 ul of recombinant FKBPL protein with 1 ug in assay buffer. 3.Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4.Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5.Record the increase in A405 nm for 30 minutes at 25°C. |
| General Readings | McKeen HD., et al. (2008) Endocrinology 149: 5724. Jascur T., et al (2005) Mol. Cell 17 (2): 237–49. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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