Product Description for Peroxiredoxin-4 / PRDX4
Peroxiredoxin-4 / PRDX4.
Presentation: Purified
Presentation: Purified
Properties for Peroxiredoxin-4 / PRDX4
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | AOE37-2, Antioxidant enzyme AOE372, PRDX-4, Prx-4, Prx-IV, Prx4, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372 |
| Presentation | Purified |
| Molecular weight | 28.8 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
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| Purity | > 95 % by SDS - PAGE |
| Background | PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay: 1.Prepare a 50ul reaction mix into a suitable container : The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2.Add 5 ul of recombinant PRDX4 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3.Incubate at 25°C for 2 minutes. 4.Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5.Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6.Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) 7.Record the increase in A475nm. |
| General Readings | Wang HQ., et al. (2009) FEBS Lett. 583(9):1511-5. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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