Product Description for Cyclophilin G / PPIG
Cyclophilin G / PPIG.
Presentation: Purified
Presentation: Purified
Properties for Cyclophilin G / PPIG
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CARS-Cyp, CARS-cyclophilin, CASP10, Clk-associating RS-cyclophilin, PPIase G, Peptidyl-prolyl cis-trans isomerase G, Peptidyl-prolyl isomerase G, Rotamase G, SR-cyclophilin, SR-cyp, SRcyp |
| Presentation | Purified |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 95 % by SDS - PAGE |
| Background | Peptidyl-prolyl cis-trans isomerase G (PPIG), also known as Cyclophilin G, is a member of peptidyl-prolyl cis-trans isomerase family (PPIases). This protein catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is localized to the nuclear speckles, a nuclear compartment rich in splicing factors, and interacts with the splicing factors SC35 and pinin. Cyclophilin G also may play an important role in the regulation of pre-mRNA splicing. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2.Add 1 ug of recombinant Cyclophilin G protein to assay buffer. 3.Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4.Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5.Record the increase in A405 nm for 30 minutes at 25°C. |
| General Readings | Modjtahedi N., et al. (2004). J Biol Chem. 279(21):22322-30. Ouyang P., et al. (2004). Biochem Biophys Re Commun. 321 (3): 638–47. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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