Product Description for PPIL1
PPIL1.
Presentation: Purified
Presentation: Purified
Properties for PPIL1
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | CYPL1, PPIase, Peptidyl-prolyl cis-trans isomerase-like 1, Rotamase PPIL1 |
| Presentation | Purified |
| Molecular weight | 19.3 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 95 % by SDS PAGE |
| Background | Peptidylprolyl isomerase (cyclophilin)-like 1, also known as PPIL1, is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. Human PPIL1 might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. So it is expected as a novel molecular target for colon-cancer therapy. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2.Add 10 ul of recombinant PPIL1 protein with 1 ug in assay buffer. 3.Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4.Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5.Record the increase in A405 nm for 30 minutes at 25°C. |
| General Readings | Xu C., et al., (2006) J Biol Chem. 281(23):15900-8. Obama K., et al., (2006) Clin Cancer Res. 12(1):70-6. |
| Storage | Store (in aliquots) at -20°C or -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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