NEDD8 (1-76, His-tag) - AR09126PU-N

Product Description for NEDD8

NEDD8.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.1 mg
Synonyms	Neural precursor cell expressed developmentally down-regulated protein 8
Presentation	Purified
Molecular weight	12.8 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: Q15843
Purity	> 95 % by SDS - PAGE
Background	NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Concentration	0.5 mg/ml (determined by Bradford assay)
General Readings	Wu K., et al. (2002) J Biol Chem. 277(1): 516-27.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human NEDD8 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer. AA Sequence: `MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG` Molecular weight: (113 aa)
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.5 mM DTT, 50 mM NaCl, 10% glycerol Endotoxin level: < 1.0 EU per 1 μg of protein (determined by LAL method) Purity: >95% by SDS - PAGE State: Liquid purified protein Purified