AR09154PU-N AKR1C3 / DDH3 (1-323, His-tag)

Search for all "AKR1C3 / DDH3"

0.1 mg / €320.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

AKR1C3 / DDH3

Product Description for AKR1C3 / DDH3

AKR1C3 / DDH3.
Presentation: Purified

Properties for AKR1C3 / DDH3

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms 17-beta-hydroxysteroid dehydrogenase type 5, 2-dihydrobenzene-1, 2-diol dehydrogenase, 3-alpha-HSD type II, 3-alpha-HSD type II, 3-alpha-hydroxysteroid dehydrogenase type 2, Aldo-keto reductase family 1 member C3, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase type I, HA1753, HSD17B5, KIAA0119, PGFS, Prostaglandin F synthase, Testosterone 17-beta-dehydrogenase 5, Trans-1, brain
Presentation Purified
Molecular weight 39 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P42330
Purity > 95 % by SDS - PAGE
Background AKR1C3, also known as PGFS, is a member of the aldo-keto reductase superfamily which catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9 alpha, 11 beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and differentiation.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings
  1. Davies NJ, Hayden RE, Simpson PJ, Birtwistle J, Mayer K, Ride JP, et al. AKR1C isoforms represent a novel cellular target for jasmonates alongside their mitochondrial-mediated effects. Cancer Res. 2009 Jun 1;69(11):4769-75. doi: 10.1158/0008-5472.CAN-08-4533. PubMed PMID: 19487289.
  2. Kabututu Z, Manin M, Pointud JC, Maruyama T, Nagata N, Lambert S, et al. Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7. J Biochem. 2009 Feb;145(2):161-8. doi: 10.1093/jb/mvn152. Epub 2008 Nov 14. PubMed PMID: 19010934.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human AKR1C3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY
Biological activity:
Specific activity is > 1,000 pmol/min/µg, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole 1-Acenaphthenol presence of NADP per minute at pH 8.8 at 25°C.
Molecular weight:
(343 aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Endotoxin level:
< 1.0 EU per 1 µg of protein (determined by LAL method)
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
  • LinkedIn