BM2145 Beta-2-microglobulin antibody

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0.2 mg / €700.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Beta-2-microglobulin B1G6

BM2145

Product Description for Beta-2-microglobulin

Mouse anti Human Beta-2-microglobulin B1G6.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for Beta-2-microglobulin

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms B2M, Beta 2 microglobulin, Beta-2 microglobulin
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF
Clonality Monoclonal
Clone B1G6
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P61769
Immunogen:
Purified human Beta 2-Microglobulin
GeneID:
567
Isotype control AM03096PU-N
Application Purification of the HLA antigens associated with Beta 2-Microglobulin.
Fluorescence Microscopy or Flow Cytometry: 2 µl/5x105 cells/test.
Immunohistochemistry on Frozen Sections or Cell Smears: Working dilution 1/25-1/50.
Background Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Concentration 0.2 mg/ml (prior to lyophilization)
General Readings
  1. Liabeuf A, le Borgne de Kaouel C, Kourilsky FM, Malissen B, Manuel Y, Sanderson AR. An antigenic determinant of human beta 2-microglobulin masked by the association with HLA heavy chains at the cell surface: analysis using monoclonal antibodies. J Immunol. 1981 Oct;127(4):1542-8. PubMed PMID: 6168695.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer. 
Addition of 0.09% (w/v) Sodium Azide is recommended for storage up to one month at 2-8°C. 
Avoid repeated freezing and thawing. 
Shelf life: one year from despatch. 

Format
Purification:
Protein A Chromatography
Buffer System:
PBS
Preservatives:
None
Stabilizers:
1 mg/ml BSA
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with 1.0 ml distilled water
Specificity
Specificity:
Specific for Human beta 2-Microglobulin (MW 12 kDa).
Recognizes the HLA-beta 2-Microglobulin complex, free or associated with the membrane. Does not react with other species.
The exact epitope for the antibody has not been mapped.
Gene ID 567

Accessory Products

Proteins and/or Positive Controls

Proteins for Beta-2-microglobulin (21 products)

Catalog No. Species Pres. Purity   Source  

Beta-2-microglobulin

Beta-2-microglobulin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Beta-2-microglobulin (C-term DDK tag)

Beta-2-microglobulin Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

Beta-2-microglobulin

Beta-2-microglobulin Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Beta-2-microglobulin (21-119, His-tag)

Beta-2-microglobulin Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €820.00
  OriGene Technologies GmbH

Beta-2-microglobulin (21-119, His-tag)

Beta-2-microglobulin Human Purified > 95 % by SDS - PAGE E. coli
10 µg / €320.00
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95.0% as determined by by both RP-HPLC and SDS-PAGE E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95.0% as determined by by both RP-HPLC and SDS-PAGE E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95.0% as determined by by both RP-HPLC and SDS-PAGE E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Beta-2-microglobulin

Beta-2-microglobulin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  Alpha Diagnostic Intl. Inc.

Beta-2-microglobulin

Beta-2-microglobulin Human > 98 % Urine
  BBI Solutions

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 40 % Urine
  BBI Solutions

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  Novus Biologicals Inc.

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  Novus Biologicals Inc.

Positive controls for Beta-2-microglobulin (1 products)

Catalog No. Species Pres. Purity   Source  

beta 2 Microglobulin Lysate

Western Blot: beta 2 Microglobulin Lysate [NBL1-07874] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for B2M Protein
  Novus Biologicals Inc.
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