BP1021B E. coli (O+K antigens) antibody

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1 ml / €610.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti E. coli

BP1021B

Product Description for E. coli

Rabbit anti E. coli.
Properties: (O+K antigens)
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for E. coli

Product Category Primary Antibodies
Quantity 1 ml
Synonyms EHEC, EPEC, ETEC, Escherichia coli
Presentation Biotin
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mixture of E. coli serotypes
Property O+K antigens
Application Avidin and streptavidin amplification systems for ELISA and Fluorescence Microscopy.
Has been described for previous lots to work in Western blot.
Background Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Concentration 4-5 mg/ml (OD280 nm, E0.1% = 1.4)
General Readings
  1. Salminen A, Loimaranta V, Joosten JA, Khan AS, Hacker J, Pieters RJ, et al. Inhibition of P-fimbriated Escherichia coli adhesion by multivalent galabiose derivatives studied by a live-bacteria application of surface plasmon resonance. J Antimicrob Chemother. 2007 Sep;60(3):495-501. Epub 2007 Jul 10. PubMed PMID: 17623698.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Covalently coupled with the N-Hydroxysuccinimide ester of under mild conditions to give a high degree of substitution
Biotin
Purification:
Protein A Chromatography.
Buffer System:
0.01 M PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and no stabilizers.
State:
Liquid purified IgG fraction.
Specificity
Specificity:
Many O and K antigenic serotypes of E. coli.
Not absorbed and does cross-react with related Enterobacteriaceae.
Will remove E.coli proteins from recombinant preparations.

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