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BP2056HRP Apolipoprotein B / Apo B antibody

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1 ml / €870.00
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Quick Overview

Goat anti Human Apolipoprotein B / Apo B

BP2056HRP

Product Description for Apolipoprotein B / Apo B

Goat anti Human Apolipoprotein B / Apo B.
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Apolipoprotein B / Apo B

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Apo-B, ApoB, ApoB-100, ApoB100
Presentation HRP
Reactivity Hu
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P04114
Immunogen:
Human LDL.
GeneID:
338
Application Can be used to detect the existence of Apo B-48/100 in plasma and lipoproteins for Immunoassay and Immunoblot.
Dilution range for Immunoblot and ELISA: 1/2,500-1/10,000.
Background Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/l.
Caution Use of Sodium Azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Concentration 1.0 mg/ml (prior to lyophilization)
General Readings
  1. Ng CJ, Bourquard N, Grijalva V, Hama S, Shih DM, Navab M, et al. Paraoxonase-2 deficiency aggravates atherosclerosis in mice despite lower apolipoprotein-B-containing lipoproteins: anti-atherogenic role for paraoxonase-2. J Biol Chem. 2006 Oct 6;281(40):29491-500. Epub 2006 Aug 4. PubMed PMID: 16891303.
  2. Herscovitz H, Derksen A, Walsh MT, McKnight CJ, Gantz DL, Hadzopoulou-Cladaras M, et al. The N-terminal 17% of apoB binds tightly and irreversibly to emulsions modeling nascent very low density lipoproteins. J Lipid Res. 2001 Jan;42(1):51-9. PubMed PMID: 11160365.
  3. Kitchens RL, Thompson PA, Munford RS, O'Keefe GE. Acute inflammation and infection maintain circulating phospholipid levels and enhance lipopolysaccharide binding to plasma lipoproteins. J Lipid Res. 2003 Dec;44(12):2339-48. Epub 2003 Aug 16. PubMed PMID: 12923224.
  4. Lin MC, Gordon D, Wetterau JR. Microsomal triglyceride transfer protein (MTP) regulation in HepG2 cells: insulin negatively regulates MTP gene expression. J Lipid Res. 1995 May;36(5):1073-81. PubMed PMID: 7658155.
  5. Reardon CA, Miller ER, Blachowicz L, Lukens J, Binder CJ, Witztum JL, et al. Autoantibodies to OxLDL fail to alter the clearance of injected OxLDL in apolipoprotein E-deficient mice. J Lipid Res. 2004 Jul;45(7):1347-54. Epub 2004 Apr 21. PubMed PMID: 15102879.
  6. Schneider M, Witztum JL, Young SG, Ludwig EH, Miller ER, Tsimikas S, et al. High-level lipoprotein [a] expression in transgenic mice: evidence for oxidized phospholipids in lipoprotein [a] but not in low density lipoproteins. J Lipid Res. 2005 Apr;46(4):769-78. Epub 2005 Jan 16. PubMed PMID: 15654123.
Storage Store the lyophilized antibody at 2-8°C.
Store the antibody after reconstitution for several weeks at 2-8°C.
Prepare working dilution only prior to immediate use.
For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20°C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Human Apolipoprotein B-100 Sepharose Affinity Chromatography.
Buffer System:
50 mM PBS, 0.1M Sodium Chloride, pH 7.4 containing 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml distilled water.
Centrifuge product if not completely clear after standing for 1-2 hours at room temperature.
Specificity
Specificity:
Binds to Human Apo B-48/100.
No cross- reaction with human Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII and Apo E.
Gene ID 338

Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein B / Apo B (7 products)

Catalog No. Species Pres. Purity   Source  

Apolipoprotein B / Apo B
Apolipoprotein B / Apo B Human Purified > 95 % Column chromatography.
single arc by IEP against whole human serum. 		Plasma
0.5 mg / €260.00
  OriGene Technologies GmbH

Apolipoprotein B / Apo B (APOB 100)
Apolipoprotein B / Apo B Human Purified Method of Purification:
1. Ultracentrifugation at d 1.03-1.05
2. Delipidation with Diethyl Ether/Ethanol (3/1)
3. Gel filtration over Sephacryl S-200. 		Plasma
1 mg / €330.00
  OriGene Technologies GmbH

Apolipoprotein B / Apo B
Apolipoprotein B / Apo B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Apolipoprotein B / Apo B
Apolipoprotein B / Apo B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Apolipoprotein B / Apo B
Apolipoprotein B / Apo B Human
  Abnova Taiwan Corp.

Apolipoprotein B / Apo B
Apolipoprotein B / Apo B Human
  Abnova Taiwan Corp.

Apolipoprotein B / Apo B (ApoB 100)
Apolipoprotein B / Apo B Human Purified > 80 % E. coli
  Genesis Biotech Inc.

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