RAF1 - AP00041PU-N | acris-antibodies.com

Product Description for RAF1

Rabbit anti Human, Mouse, Rat RAF1.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for RAF1

Product Category	Primary Antibodies
Quantity	0.1 mg
Synonyms	C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Presentation	Aff - Purified
Reactivity	Hu, Ms, Rt
Applications	IP, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P04049 Immunogen: Synthetic peptide mapping to the C-terminus of Human Raf-1. GeneID: 5894
Application	Western Blot: 1-4 μg/ml. Immunoprecipitation: 15-25 μg/ml. Immunohistochemistry: 10-20 μg/ml.
Background	Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
Concentration	0.2 mg/ml
Storage	Store undiluted at -20°C or for long term storage (in aliquots) at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Immunoaffinity Chromatography Buffer System: PBS, pH 7.2 containing 50% glycerol, 1% BSA, 0.02% Thimerosal State: Liquid purified IgG fraction Aff - Purified
Specificity	Specificity: The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is not cross-reactive with Raf-A and Raf-B. Species: Human, Mouse, Rat.
Gene ID	5894

Accessory Products

Proteins and/or Positive Controls

Proteins for RAF1 (26 products)

Catalog No.	Species	Pres.	Purity	Source
TP301983	RAF1
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
H00005894-P01	RAF1
	Human	Purified
					Abnova Taiwan Corp.
H00005894-P01-25	RAF1
	Human	Purified
					Abnova Taiwan Corp.
H00005894-Q01-10	RAF1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00005894-Q01-25	RAF1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P1449	RAF1
	Human
					Abnova Taiwan Corp.
P1485	RAF1
	Human
					Abnova Taiwan Corp.
P1487	RAF1
	Human
					Abnova Taiwan Corp.
P1498	RAF1
	Human
					Abnova Taiwan Corp.
P1578	RAF1
	Human
					Abnova Taiwan Corp.
P1580	RAF1
	Human
					Abnova Taiwan Corp.
P1594	RAF1
	Human
					Abnova Taiwan Corp.
P1609	RAF1
	Human
					Abnova Taiwan Corp.
P1644	RAF1
	Human
					Abnova Taiwan Corp.
P2830	RAF1
	Human
					Abnova Taiwan Corp.
P2834	RAF1
	Human
					Abnova Taiwan Corp.
P2835	RAF1
	Human
					Abnova Taiwan Corp.
P2836	RAF1
	Human
					Abnova Taiwan Corp.
P2837	RAF1
	Human
					Abnova Taiwan Corp.
P2838	RAF1
	Human
					Abnova Taiwan Corp.
P2839	RAF1
	Human
					Abnova Taiwan Corp.
P4210	RAF1
	Human
					Abnova Taiwan Corp.
P4836	RAF1
	Human
					Abnova Taiwan Corp.
NBP1-74359	RAF1
	Human	Purified
					Novus Biologicals Inc.
NBP1-74360	RAF1
	Human	Purified
					Novus Biologicals Inc.
NBP1-74361	RAF1
	Human	Purified
					Novus Biologicals Inc.

Positive controls for RAF1 (1 products)

Catalog No.	Species
H00005894-T01	RAF1 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.

Recommended Secondary Antibodies for RAF1 (9 products)

Catalog No.	Host	Pres.	React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP	Rb	E, WB
					OriGene Technologies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin	Rb	E, WB
					OriGene Technologies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC	Rb	E, ICC/IF, WB
					OriGene Technologies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP	Rb	E, P, WB
					OriGene Technologies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC	Rb	E, F, ICC/IF
					OriGene Technologies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE	Rb	F
					OriGene Technologies GmbH
R1458C2	Rabbit IgG (H+L chain, adsorbed) antibody
	Goat	Cy2
					OriGene Technologies GmbH
R1458C3	Rabbit IgG (H+L chain, adsorbed) antibody
	Goat	Cy3
					OriGene Technologies GmbH
R1458C5	Rabbit IgG (H+L chain, adsorbed) antibody
	Goat	Cy5
					OriGene Technologies GmbH

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AP00041PU-N RAF1 antibody

Quick Overview

Product Description for RAF1

Properties for RAF1

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for RAF1 (26 products)

Positive controls for RAF1 (1 products)

Recommended Secondary Antibodies for RAF1 (9 products)

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