EIF4E (1-217, His-tag) - AR09601PU-L

Product Description for EIF4E

EIF4E.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.25 mg
Synonyms	EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
Presentation	Purified
Molecular weight	27.2 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P06730
Purity	> 85 % by SDS - PAGE
Background	EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Concentration	0.5 mg/ml (determined by Bradford assay)
General Readings	Bendahmane A., et al. (2010) PLoS One. 5(6):e11313. Pelletier J., et al. (2010) J Gen Virol. 91(Pt 5):1224-8.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV` Molecular weight: (237aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol Purity: >85% by SDS - PAGE State: Liquid purified protein Purified