Product Description for MDH1
MDH1.
Presentation: Purified
Presentation: Purified
Properties for MDH1
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | MDHA, Malate dehydrogenase, cytoplasmic |
| Presentation | Purified |
| Molecular weight | 37.4 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
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|---|---|
| Purity | > 95 % by SDS-PAGE |
| Background | MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 100 mM Potassium phosphate, 0.13 mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25 mM oxalacetic acid. 2.Add recombinant MDH protein with various concentrations (0.1ug, 0.5ug, 1ug) in assay buffer. 3.Mix by inversion, equilibrate to 1°C, and monitor at A340nm until the value is constant using a spectrophotometer. 4.Record the increase at A340nm for approximately 2 minutes. |
| General Readings | Youn HD., et al. (2009) Cell Death Differ. 16(5):738-48 Waye MM., et al (2005) J Cell Biochem. 94(4):763-73. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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