AR09614PU-N Thiamine-triphosphatase (THTPA) (1-230, His-tag)

Search for all "Thiamine-triphosphatase (THTPA)"

0.1 mg / €320.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Thiamine-triphosphatase (THTPA)

AR09614PU-N

More Views

  • AR09614PU-N

Product Description for Thiamine-triphosphatase (THTPA)

Thiamine-triphosphatase (THTPA).
Presentation: Purified

Properties for Thiamine-triphosphatase (THTPA)

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms ThTPase
Presentation Purified
Molecular weight 27.7 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9BU02
Purity > 90 % by SDS – PAGE
Background THTPA, also known as THTP or THTPASE, is a member of the THTPase family. This protein is localized to the cytoplasm and expressed at low levels in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the major form of thiamine within the cell. THTPA exists as a monomer and functions at an optimal pH of 8.5.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Lakaye B., et al. (2002) J Biol Chem. 277(16):13771-7.
Lakaye B., et al. (2004) Int J Biochem Cell Biol. 36(7):1348-64.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human THTPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAQGLIEVER KFLPGPGTEE RLQELGGTLE YRVTFRDTYY DTPELSLMQA DHWLRRREDS GWELKCPGAA GVLGPHTEYK ELTAEPTIVA QLCKVLRADG LGAGDVAAVL GPLGLQEVAS FVTKRSAWKL VLLGADEEEP QLRVDLDTAD FGYAVGEVEA LVHEEAEVPT ALEKIHRLSS MLGVPAQETA PAKLIVYLQR FRPQDYQRLL EVNSSRERPQ ETEDPDHCLG
Molecular weight:
(250aa) confirmed by MALDI-TOF (Real molecular weight on SDS-PAGE will be shift up).
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT
Purity:
>90% by SDS – PAGE
State:
Liquid purified protein
Purified
  • LinkedIn