Product Description for APG5L / ATG5
Mouse anti Canine, Human, Mouse, Rat APG5L / ATG5 7C6.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.
Properties for APG5L / ATG5
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5 |
| Presentation | Purified |
| Reactivity | Can, Hu, Ms, Rt |
| Applications | F, ICC/IF, WB |
| Clonality | Monoclonal |
| Clone | 7C6 |
| Host | Mouse |
| Isotype | IgG1 |
| Molecular weight | 24, 33, 55 kDa |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples) |
| Application | Flow Cytometry. Immunobotting (Western Blot): 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blot incubation buffer. Immunocytochemistry: 0.5 µg/ml. Included Postitive Control: Cell lysate from untreated SH-SY5Y cells (See Protocols). |
| Background | Autophagy as a response to cellular stress and starvation is an alternative process of proteasomal degradation for some long-lived proteins and organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). The gene product of autophagy-related gene 5 (ATG5) is required for autophagosome-formation. ATG5 also enhances the susceptibility towards apoptotic stimuli. Like Bcl-2, ATG5 exhibits a dual function by modulating both autophagy and apoptosis. |
| Protocols | Positive Control: Cell lysate from untreated SH-SY5Y cells. Formulation: Lyophilized cell lysate from Serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of Positive Control correspond to ca. 20.000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 9474 |
Accessory Products
Proteins and/or Positive Controls
Proteins for APG5L / ATG5 (14 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP310563 |
APG5L / ATG5 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR09843PU-L |
APG5L / ATG5 (1-275, His-tag) |
||||||
|
Human | Purified | > 85 % | E. coli |
0.25 mg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09843PU-N |
APG5L / ATG5 (1-275, His-tag) |
||||||
|
|
Human | Purified | > 85 % | E. coli |
50 µg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| AR10328PU-N |
APG5L / ATG5 (T7-tagged) |
||||||
|
Human | Purified | > 95.0% as determined by SDS-PAGE | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10328PU-S |
APG5L / ATG5 (T7-tagged) |
||||||
|
|
Human | Purified | > 95.0% as determined by SDS-PAGE | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10388PU-L |
APG5L / ATG5 (T7-tagged) |
||||||
|
|
Human | Purified | > 95.0% as determined by SDS-PAGE | E. coli | |||
| OriGene Technologies GmbH | |||||||
| H00009474-P01-10 |
APG5L / ATG5 |
||||||
|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00009474-P01-25 |
APG5L / ATG5 |
||||||
|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00009474-Q01-10 |
APG5L / ATG5 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00009474-Q01-25 |
APG5L / ATG5 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| 10-288-22210F |
APG5L / ATG5 |
||||||
|
|
Human | Purified | 95 % | E. coli | |||
| GenWay Biotech Inc. | |||||||
| 10-288-22210F-50 |
APG5L / ATG5 |
||||||
|
|
Human | Purified | 95 % | E. coli | |||
| GenWay Biotech Inc. | |||||||
| NBP1-50982-0.25 |
APG5L / ATG5 |
||||||
|
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| NBP1-50982-50 |
APG5L / ATG5 |
||||||
|
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
Positive controls for APG5L / ATG5 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07799 |
ATG5 Lysate |
||||||
![Western Blot: ATG5 Lysate [NBL1-07799] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for ATG5](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/ATG5%20Lysate-Western%20Blot-NBL1-07799-img0001.jpg) |
Protein | ||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for APG5L / ATG5 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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