AR09658PU-N LYPLA1 (1-230, His-tag)

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0.1 mg / €320.00
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LYPLA1

AR09658PU-N

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  • AR09658PU-N

Product Description for LYPLA1

LYPLA1.
Presentation: Purified

Properties for LYPLA1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms APT-1, APT1, Acyl-protein thioesterase 1, LPL-1, LPL1, LysoPLA I, Lysophospholipase 1
Presentation Purified
Molecular weight 26.8 kDa
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P97823
Purity > 90 % by SDS - PAGE
Background LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Schaloske R.H., et al. (2006) Biochim Biophys Acta. 1761:1246-1259.
Hiraoka M., et al. (2002) J. Biol. Chem 277:10090-10099.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID
Molecular weight:
(250aa), confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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