Product Description for PTK6 / BRK
Mouse anti Human PTK6 / BRK 24B7.
Presentation: Purified
Product is tested for Western blot / Immunoblot.
Presentation: Purified
Product is tested for Western blot / Immunoblot.
Properties for PTK6 / BRK
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Breast tumor kinase, Tyrosine-protein kinase 6, Tyrosine-protein kinase BRK |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | WB |
| Clonality | Monoclonal |
| Clone | 24B7 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Add. information | Molecular Weight: 52 kDa |
| Application | Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols for more details). |
| Background | This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation. |
| Concentration | 0.1 mg/ml |
| Protocols | Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma (Human) Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing. |
| Storage | Store the antibody in aliquots) at -20°C. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 5753 |
Accessory Products
Proteins and/or Positive Controls
Proteins for PTK6 / BRK (8 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP307766 |
PTK6 / BRK |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| H00005753-P01 |
PTK6 / BRK |
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|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00005753-P01-25 |
PTK6 / BRK |
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|
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00005753-Q01-10 |
PTK6 / BRK |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005753-Q01-25 |
PTK6 / BRK |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P2759 |
PTK6 / BRK |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4192 |
PTK6 / BRK |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4637 |
PTK6 / BRK |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
Positive controls for PTK6 / BRK (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-14954 |
Brk Lysate |
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![Western Blot: Brk Lysate [NBL1-14954] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for PTK6](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Brk%20Lysate-Western%20Blot-NBL1-14954-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
| H00005753-T01 |
PTK6 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| LY416957 |
PTK6 overexpression lysate |
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|
0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for PTK6 / BRK (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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