Product Description for Cadherin-2
Mouse anti Human, Mouse Cadherin-2 7E9.
Properties: (incl. pos. control)
Presentation: Biotin
Product is tested for Western blot / Immunoblot.
Properties: (incl. pos. control)
Presentation: Biotin
Product is tested for Western blot / Immunoblot.
Properties for Cadherin-2
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CDH2, CDHN, CDw325, Cadherin N, N-Cadherin, NCAD, Neural cadherin |
| Presentation | Biotin |
| Reactivity | Hu, Ms |
| Applications | WB |
| Clonality | Monoclonal |
| Clone | 7E9 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | SM10B (for use in human samples) |
| Application | Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for more details). |
| Background | Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin. |
| Concentration | 0.5 mg/ml |
| Protocols | Positive Control: Cell lysate from untreated SH-SY5Y cells Format: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing. |
| Storage | Aliquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Format |
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| Specificity |
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| Gene ID | 1000 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cadherin-2 (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP307170 |
Cadherin-2 |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||||
| H00001000-G01 |
Cadherin-2 |
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Human | ||||||
| Abnova Taiwan Corp. | |||||||
| H00001000-H01 |
Cadherin-2 |
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Human | Human HEK293T cells | |||||
| Abnova Taiwan Corp. | |||||||
| H00001000-Q01-10 |
Cadherin-2 |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001000-Q01-25 |
Cadherin-2 |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Cadherin-2 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-09030 |
N Cadherin Lysate |
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![Western Blot: N Cadherin Lysate [NBL1-09030] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for CDH2](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/N%20Cadherin%20Lysate-Western%20Blot-NBL1-09030-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Cadherin-2 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
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Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
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Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
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|
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Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
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|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
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Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
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Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
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Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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