Product Description for Adenylate kinase 1 / AK1
Adenylate kinase 1 / AK1.
Presentation: Purified
Presentation: Purified
Properties for Adenylate kinase 1 / AK1
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | ATP-AMP transphosphorylase 1, Myokinase |
| Presentation | Purified |
| Molecular weight | 23.7 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 95 % |
| Background | AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1. Prepare 1.4 ml assay buffer (Assay buffer: 58 mM glycylglycine, 2.0 mM adenosine 5'-diphosphate, 2.3 mM beta-nicotinamide adenine dinucleotide phosphate, 10 mM magnesium chloride, 10 mM glucose, 2 unit hexokinase, 1 unit glucose-6-phosphate dehydrogenase, 0.003% (w/v) BSA, pH 7.5) - G-6-PDH/Hex (sigma,Cat.No. H-8629) 2. Add 50 ul of recombinant AK1 protein with various concentrations (0.5ug, 1ug) and read the increase in A340nm for 5 minutes. |
| General Readings | Terzic A., et al. (2007) J Biol Chem. 282(43):31366-72. Morelli A., et al (2007) Curr Eye Res. 32(3):249-57. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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