PFDN1 (14-122, His-tag) - AR09709PU-S

Product Description for PFDN1

PFDN1.
Presentation: Purified

Immunogen	Swiss Prot Num: O60925
Purity	> 90 %
Background	PFDN1 is one of sixsubunits of prefoldin, a heterohexameric chaperone protein which assists in the correct folding of other proteins. This protein delivers nonnative target proteins, principally actins and tubulins, to the eukaryotic cytosolic chaperonin for facilitated folding. Defects in PFDN1 displayed phenotypes characteristic of defects in cytoskeletal function, including manifestations of ciliary dyskinesia, neuronal loss, and defects in B and T cell development and function.
Concentration	0.5 mg/ml (determined by Bradford assay)
General Readings	Ruley HE., et al. (2008) J Immunol. 181(1):476-84. Cowan NJ., et al (2004) J Biol Chem. 279(6):4196-203.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human PFDN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MTELQAKVID TQQKVKLADI QIEQLNRTKK HAHLTDTEIM TLVDETNMYE GVGRMFILQS KEAIHSQLLE KQKIAEEKIK ELEQKKSYLE RSVKEAEDNI REMLMARRAQ` Molecular weight: (130aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl Buffer (pH 8.0) containing 20% Glycerol Purity: >90% State: Liquid purified protein Purified