AP21307PU-N Glutathione reductase antibody

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1 mg / €530.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Bakers Yeast Glutathione reductase

AP21307PU-N

Product Description for Glutathione reductase

Rabbit anti Bakers Yeast Glutathione reductase.
Presentation: Aff - Purified
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Glutathione reductase

Product Category Primary Antibodies
Quantity 1 mg
Synonyms GLUR, GR, GRD1, GRase, GSR
Presentation Aff - Purified
Reactivity Bakers Yeast
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P41921
Immunogen:
Glutathione Reductase isolated and purified from Baker's Yeast.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
856014
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
the customer’s own choice.
Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/600.
Background Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.
Concentration 2.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Format
Purification:
Solid Phase Affinity Chromatography
Buffer System:
PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
State:
Lyophilized purified IgG fraction
Aff - Purified
Reconstitution:
Restore by adding 0.5 ml of sterile distilled water
Specificity
Specificity:
The antibody recognizes Glutathione Reductase from Baker’s yeast.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Species:
Baker’s Yeast.
Gene ID 856014

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