Product Description for MDH1
Rabbit anti Human MDH1.
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.
Properties for MDH1
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 10 mg |
| Synonyms | MDHA, Malate dehydrogenase, cytoplasmic |
| Presentation | Azide Free |
| Reactivity | Hu |
| Applications | Dot, E, ICC/IF, ID, IP, R, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Application | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/3,000. |
| Background | Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase. |
| Concentration | 10.0 mg/ml |
| Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 4190 |
Accessory Products
Proteins and/or Positive Controls
Proteins for MDH1 (11 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP300298 |
MDH1 |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| TP720248 |
MDH1 (transcript variant 1) |
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|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR09603PU-L |
MDH1 (1-334, His-tag) |
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|
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.5 mg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09603PU-N |
MDH1 (1-334, His-tag) |
||||||
|
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.1 mg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| H00004190-P01-10 |
MDH1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00004190-P01-25 |
MDH1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00004190-Q01-10 |
MDH1 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00004190-Q01-25 |
MDH1 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| P3512 |
MDH1 |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| NBP1-45315-0.1 |
MDH1 |
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|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
| NBP1-45315-0.5 |
MDH1 |
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|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
Positive controls for MDH1 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-12964 |
MDH1 Lysate |
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![]() |
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| Novus Biologicals Inc. | |||||||
| LY434177 |
MDH1 overexpression lysate |
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![]() |
0.1 mg /
€315.00
|
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| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for MDH1 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
![Western Blot: MDH1 Lysate [NBL1-12964] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for MDH1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/MDH1%20Lysate-Western%20Blot-NBL1-12964-img0001.jpg)
