AP21344AF-N MDH1 antibody

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10 mg / €230.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human MDH1

AP21344AF-N

Product Description for MDH1

Rabbit anti Human MDH1.
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for MDH1

Product Category Primary Antibodies
Quantity 10 mg
Synonyms MDHA, Malate dehydrogenase, cytoplasmic
Presentation Azide Free
Reactivity Hu
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P40925
Immunogen:
Malic dehydrogenase is isolated and purified from Human placenta.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
4190
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
the customer’s own choice.
Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/3,000.
Background Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.
Concentration 10.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
State:
Lyophilized IgG fraction
Azide Free
Reconstitution:
Restore by adding 1.0 ml of sterile distilled water
Specificity
Specificity:
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined
Species:
Human.
Gene ID 4190

Accessory Products

Proteins and/or Positive Controls

Proteins for MDH1 (11 products)

Catalog No. Species Pres. Purity   Source  

MDH1

MDH1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

MDH1 (transcript variant 1)

MDH1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

MDH1 (1-334, His-tag)

MDH1 Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

MDH1 (1-334, His-tag)

MDH1 Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

MDH1

MDH1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MDH1

MDH1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MDH1

MDH1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

MDH1

MDH1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

MDH1

MDH1 Human
  Abnova Taiwan Corp.

MDH1

MDH1 Human Purified
  Novus Biologicals Inc.

MDH1

MDH1 Human Purified
  Novus Biologicals Inc.

Positive controls for MDH1 (2 products)

Catalog No. Species Pres. Purity   Source  

MDH1 Lysate

Western Blot: MDH1 Lysate [NBL1-12964] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MDH1
  Novus Biologicals Inc.

MDH1 overexpression lysate

MDH1 overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.
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