AP21360PU-N Horseradish Peroxidase / HRP antibody

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1 mg / €530.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Horsereddish Horseradish Peroxidase / HRP

AP21360PU-N

Product Description for Horseradish Peroxidase / HRP

Rabbit anti Horsereddish Horseradish Peroxidase / HRP.
Presentation: Aff - Purified
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Horseradish Peroxidase / HRP

Product Category Primary Antibodies
Quantity 1 mg
Presentation Aff - Purified
Reactivity Horsereddish
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Peroxidase isolated and purified from Horseradish.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
the customer’s own choice.
Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
Background Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems.
HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Concentration 2.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Format
Purification:
Solid Phase Affinity Chromatography
Buffer System:
PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
State:
Lyophilized purified IgG fraction
Aff - Purified
Reconstitution:
Restore by adding 0.5 ml of sterile distilled water
Specificity
Specificity:
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.

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