AP21407BT-N GOT1 antibody

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1 ml / €390.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Porcine GOT1

AP21407BT-N

Product Description for GOT1

Rabbit anti Porcine GOT1.
Presentation: Biotin
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for GOT1

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Aspartate aminotransferase, Glutamate oxaloacetate transaminase 1, Transaminase A
Presentation Biotin
Reactivity Por
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00503
Immunogen:
Glutamate-Oxaloacetate Transaminase isolated and purified from Porcine heart.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
396967
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
Recommended Dilutions:
Non-precipitating antibody-binding techniques: 1/100-1/1,000.
Background Glutamate oxaloacetate transaminase is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
Concentration 10.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Format
Label:
Biotin
Molar radio:
Biotin/IgG ~ 5.7
Purification:
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
State:
Lyophilized hyperimmune IgG fraction
Reconstitution:
Restore by adding 1.0 ml of sterile distilled water
Specificity
Specificity:
Glutamate-Oxaloacetate Transaminase from Porcine heart.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Species:
Pig.
Gene ID 396967

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