Cytokeratin 8 - BM5045P | acris-antibodies.com

Product Description for Cytokeratin 8

Mouse anti Human, Mammalia, Mouse, Rabbit, Rat Cytokeratin 8 M20.
Presentation: Purified
Product is tested for Flow Cytometry, Paraffin Sections, Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Cytokeratin 8

Product Category	Primary Antibodies
Quantity	0.1 mg
Synonyms	CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
Presentation	Purified
Reactivity	Hu, Mam, Ms, Rb, Rt
Applications	C, F, ICC/IF, P, WB
Clonality	Monoclonal
Clone	M20
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P05787 Immunogen: Keratin isolated from the Human breast carcinoma cell line MCF-7 GeneID: 3856
Isotype control	AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application	Immunoblotting: 1/100-1/1000 Flow Cytometry. Immunocytochemistry. Immunohistochemistry on Frozen Tissues: 1/100-1/200 with Avidin-biotinylated Horseradish peroxidase complex ABC as detection reagent. Immunohistochemistry on Paraffin Sections.
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration	1.0 mg/ml
General Readings	Van Muijen GN, Ruiter DJ, Warnaar SO. Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest. 1987 Oct;57(4):359-69. PubMed PMID: 3669613. Van Muijen GN, Warnaar SO, Ponec M. Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res. 1987 Aug;171(2):331-45. PubMed PMID: 2442018. Schaafsma HE, Ramaekers FC, van Muijen GN, Ooms EC, Ruiter DJ. Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract. Histochemistry. 1989;91(2):151-9. PubMed PMID: 2472369. Smedts F, Ramaekers F, Robben H, Pruszczynski M, van Muijen G, Lane B, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990 Mar;136(3):657-68. PubMed PMID: 1690513. (Free PMC Article available) Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar;136(3):641-55. PubMed PMID: 1690512. (Free PMC Article available) Schaafsma HE, Ramaekers FC, van Muijen GN, Lane EB, Leigh IM, Robben H, et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol. 1990 Feb;136(2):329-43. PubMed PMID: 1689541. (Free PMC Article available) Ivanyi D, Groeneveld E, Van Doornewaard G, Mooi WJ, Hageman PC. Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis. Cancer Res. 1990 Aug 15;50(16):5143-52. PubMed PMID: 1696167. Wetzels RH, Kuijpers HJ, Lane EB, Leigh IM, Troyanovsky SM, Holland R, et al. Basal cell-specific and hyperproliferation-related keratins in human breast cancer. Am J Pathol. 1991 Mar;138(3):751-63. PubMed PMID: 1705754. (Free PMC Article available) Ku NO, Gish R, Wright TL, Omary MB. Keratin 8 mutations in patients with cryptogenic liver disease. N Engl J Med. 2001 May 24;344(21):1580-7. PubMed PMID: 11372009. Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane EB. Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry. 2004 Feb 10;43(5):1283-95. PubMed PMID: 14756564.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
Format	Buffer System: PBS Preservatives: 0.09% Sodium Azide State: Liquid purified IgG fraction Purified
Specificity	Specificity: M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of Cytokeratin 8 (See Reference 10). Species: Human, Rat, Rabbit and Mouse (most mammals).
Gene ID	3856

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 8 (17 products)

Catalog No.	Species	Pres.	Purity	Source
TP309570	Cytokeratin 8
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
AR09360PU-N	Cytokeratin 8
	Human	Purified	> 95 % determined by SDS gelelectrophoresis	E. coli	0.25 mg / €520.00
					OriGene Technologies GmbH
AR09360PU-S	Cytokeratin 8
	Human	Purified	> 95 % determined by SDS gelelectrophoresis	E. coli	0.1 mg / €360.00
					OriGene Technologies GmbH
AR50900PU-N	Cytokeratin 8 (1-483, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.5 mg / €820.00
					OriGene Technologies GmbH
AR50900PU-S	Cytokeratin 8 (1-483, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.1 mg / €320.00
					OriGene Technologies GmbH
BA1007	Cytokeratin 8
	Bovine	Purified	> 95 % (determined by SDS gelelectrophoresis)	Liver	0.25 mg / €390.00
					OriGene Technologies GmbH
BA1007S	Cytokeratin 8
	Bovine	Purified	> 95 % (determined by SDS gelelectrophoresis)	Liver	0.1 mg / €270.00
					OriGene Technologies GmbH
PA1283	Cytokeratin 8
	Human	Purified	> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained	E. coli
					OriGene Technologies GmbH
PA1283X	Cytokeratin 8
	Human	Purified	> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained	E. coli
					OriGene Technologies GmbH
PA1283XC	Cytokeratin 8
	Human	Purified	> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained	E. coli
					OriGene Technologies GmbH
PA1362	Cytokeratin 8
	Human	Purified	> 90 %	E. coli
					OriGene Technologies GmbH
PA1362X	Cytokeratin 8
	Human	Purified	> 90 %	E. coli
					OriGene Technologies GmbH
PA1362XC	Cytokeratin 8
	Human	Purified	> 90 %	E. coli
					OriGene Technologies GmbH
H00003856-P01-10	Cytokeratin 8
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00003856-P01-25	Cytokeratin 8
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00003856-Q01-10	Cytokeratin 8
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00003856-Q01-25	Cytokeratin 8
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 8 (4 products)

Catalog No.	Species
H00003856-T01	Cytokeratin-8 (KRT8) Lysate(Denatured)

		Abnova Taiwan Corp.
H00003856-T02	Cytokeratin-8 (KRT8) Lysate(Denatured)

		Abnova Taiwan Corp.
NBL1-12397	Cytokeratin 8 Lysate

		Novus Biologicals Inc.
LY419425	KRT8 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Recommended Secondary Antibodies for Cytokeratin 8 (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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BM5045P Cytokeratin 8 antibody

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