Fibrinogen - AP21437AF-N | acris-antibodies.com

Product Description for Fibrinogen

Goat anti Canine Fibrinogen.
Presentation: Azide Free
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Fibrinogen

Product Category	Primary Antibodies
Quantity	10 mg
Synonyms	FGA, FGB, FGG
Presentation	Azide Free
Reactivity	Can
Applications	ID, IP
Clonality	Polyclonal
Host	Goat
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application	Can be used as unlabelled primary or secondary antibody reagent for the indirect detection of Fibrinogen in dog cells, tissues and body fluids in Immunofluorescence and immunoenzyme methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). Recommended Dilutions: Histochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Background	Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis, releasing the fibrinopeptides A and B (molecular weight 50,000-65,000) and forming fibrin monomers. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking under the influence of factor XIII. The predominant gamma chain of normal fibrinogen (MW 50,000, with higher variants) has a low affinity for platelet binding.
Concentration	10 mg/ml
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing. Shelf life: one year from despatch.
Format	Buffer System: PBS, pH 7.2 without preservatives State: Lyophilized purified hyperimmune IgG fraction Azide Free Reconstitution: Restore by adding 1 ml of sterile distilled water.
Specificity	Specificity: The reactivity of the antiserum is restricted to Fibrinogen. In Immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified Fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. The antiserum does not cross-react with any other component of Dog plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Species: Canine.

Accessory Products

Recommended Secondary Antibodies for Fibrinogen (9 products)

Catalog No.	Host	Pres.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC	E, ICC/IF
				OriGene Technologies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1435C2	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy2
				OriGene Technologies GmbH
R1435C3	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy3
				OriGene Technologies GmbH
R1435C5	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy5	F, ICC/IF
				OriGene Technologies GmbH