Product Description for Complement C3c
Goat anti Rat Complement C3c.
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Properties for Complement C3c
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 1 ml |
| Synonyms | CPAMD1, Complement component 3 |
| Presentation | Biotin |
| Reactivity | Rt |
| Applications | C, E, ICC/IF, ID, IP, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Isotype | IgG |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Add. information | This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Application | This Biotin immunoconjugate antibody to Rat C3c is used to determine the presence and pattern of C3 in tissue lesions using Immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. In ELISA and Western blotting to identify Rat C3c in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Recommended Dilutions: Histochemistry and Cytochemistry : 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Background | C3 is the most abundant complement protein in rat serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response. |
| Concentration | 9.3 mg/ml |
| Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Shelf life: one year from despatch. |
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| Specificity |
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| Gene ID | 24232 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Complement C3c (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR10323PU-L |
Complement C3c |
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Human | Purified | > 96.0% | ||||
| OriGene Technologies GmbH | |||||||
| AR10323PU-N |
Complement C3c |
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Human | Purified | > 96.0% | ||||
| OriGene Technologies GmbH | |||||||
| AR10323PU-S |
Complement C3c |
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|
Human | Purified | > 96.0% | ||||
| OriGene Technologies GmbH | |||||||
Recommended Secondary Antibodies for Complement C3c (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1317AP |
Goat IgG (H+L chain) antibody |
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|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317B |
Goat IgG (H+L chain) antibody |
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|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317F |
Goat IgG (H+L chain) antibody |
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|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1317HRP |
Goat IgG (H+L chain) antibody |
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|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317T |
Goat IgG (H+L chain) antibody |
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|
Rabbit | TRITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1416R |
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1435C2 |
Goat IgG (H+L chain), adsorbed antibody |
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|
Donkey | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1435C3 |
Goat IgG (H+L chain), adsorbed antibody |
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|
Donkey | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1435C5 |
Goat IgG (H+L chain), adsorbed antibody |
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|
|
Donkey | Cy5 | F, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
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