AP21459TC-N Complement C3c antibody

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1 ml / €390.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human Complement C3c

AP21459TC-N

Product Description for Complement C3c

Goat anti Human Complement C3c.
Presentation: TRITC
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Complement C3c

Product Category Primary Antibodies
Quantity 1 ml
Synonyms CPAMD1, Complement component 3
Presentation TRITC
Reactivity Hu
Applications C, E, ICC/IF, ID, IP
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01024
Immunogen:
C3c is isolated and purified from pooled normal human serum.
Freund’s complete adjuvant is used in the first step of the immunization.
GeneID:
718
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application The Fluorescent immunoconjugate antibody to human C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Background C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain
Concentration 10 mg/ml
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Format
Label:
Tetramethylrhodamine Isothiocyanate
TRITC
Absorption emission:
554 nm / 573 nm
Molar radio:
Fluorescein/IgG ~1.4
Purification:
The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.
Buffer System:
PBS, pH 7.2 without preservatives
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore by adding 1 ml of sterile distilled water.
Specificity
Specificity:
In Immunoelectrophoresis against fresh Human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma.
Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Human.
Gene ID 718

Accessory Products

Proteins and/or Positive Controls

Proteins for Complement C3c (8 products)

Catalog No. Species Pres. Purity   Source  

Complement C3

Complement C3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

Complement C3

Complement C3 Human Purified > 96 % pure by SDS-PAGE
Analysis: Cellulose acetate electrophoresis shows one band only with beta-2 electrophoretic mobility. Quantitative and qualitative analysis by radial immunodiffusion.
Plasma
  OriGene Technologies GmbH

Complement C3

Complement C3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Complement C3

Complement C3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Complement C3

Complement C3 Human
  Abnova Taiwan Corp.

Complement C3

Complement C3 Human > 99 % Serum
  BBI Solutions

Complement C3

Complement C3 Human > 96 % Serum
  BBI Solutions

Complement C3b

Complement C3b Human Purified > 90 % by SDS-PAGE Serum
0.1 mg / €720.00
  OriGene Technologies GmbH
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