Fibrinogen - AP21471HR-N | acris-antibodies.com

Product Description for Fibrinogen

Goat anti Rat Fibrinogen.
Presentation: HRP
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Fibrinogen

Product Category	Primary Antibodies
Quantity	1 ml
Synonyms	FGA, FGB, FGG
Presentation	HRP
Reactivity	Rt
Applications	C, Dot, E, ICC/IF, ID, WB
Clonality	Polyclonal
Host	Goat
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: Fibrinogen is isolated from fresh plasma after removing Prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application	Can be used in Enzyme-Immunocytochemical and Immunohistochemical techniques for the detection of Fibrinogen at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemical/Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/4,000.
Background	Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.
Concentration	10 mg/ml
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing. Shelf life: one year from despatch.
Format	Label: Horseradish Peroxidase HRP Molar radio: Fluorochrome/IgG ~1.7 Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography. Buffer System: PBS, pH 7.2 without preservatives. State: Lyophilized purified IgG fraction. Reconstitution: Restore by adding 1 ml of sterile distilled water.
Specificity	Specificity: The reactivity of the antiserum is restricted to fibrinogen. In Immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified Fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with Fibrin monomers, circulating fibrinopeptides and Fibrin degradation products. The antiserum does not cross-react with any other component of rat plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Species: Rat.

Accessory Products

Recommended Secondary Antibodies for Fibrinogen (9 products)

Catalog No.	Host	Pres.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
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R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
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R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
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R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
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R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC	E, ICC/IF
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R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
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R1435C2	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy2
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R1435C3	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy3
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R1435C5	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy5	F, ICC/IF
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