AP21471SU-N Fibrinogen antibody

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1 ml / €260.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Rat Fibrinogen

AP21471SU-N

Product Description for Fibrinogen

Goat anti Rat Fibrinogen.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Fibrinogen

Product Category Primary Antibodies
Quantity 1 ml
Synonyms FGA, FGB, FGG
Presentation Serum
Reactivity Rt
Applications ID, IP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Fibrinogen is isolated from fresh plasma after removing prothrombin.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application In precipitating techniques as Immunoelectrophoresis and single or double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of Fibrinogen in Rat plasma or other body fluids or to determine its concentration.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal rat plasma in agar-block immunodiffusion titration.
Background Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot.
Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Format
State:
Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
Serum
Reconstitution:
Restore by adding 1 ml of sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is restricted to Rat fibrinogen molecule.
In Immunoelectrophoresis and Radial Immunodiffusion, using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products.
The antiserum does not cross-react with any other component of rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Rat.

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