AP21475SU-N Protein S / PROS1 antibody

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1 ml / €530.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human Protein S / PROS1

AP21475SU-N

Product Description for Protein S / PROS1

Goat anti Human Protein S / PROS1.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Protein S / PROS1

Product Category Primary Antibodies
Quantity 1 ml
Synonyms PROS, Vitamin K-dependent protein S
Presentation Serum
Reactivity Hu
Applications ID, IP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07225
Immunogen:
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
5627
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Can be used in precipitating techniques as Immunoelectrophoresis, Single or Double Radial Immunodiffusion, Electroimmunodiffusion, Immunonepholometry as well as solid phase and Neutralization reactions. As catching antibody in ELISA. To prepare an adsorbent for immunoaffinity purification of Protein S.
Plasma samples and all assay components must contain EDTA to stabilize the protein.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl Human plasma or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Single Radial Immunodiffusion: Use 1% antiserum in the gel.
Antibody Titre: Precipitin titre 1/32 when tested against in agar-block Immunodiffusion titration.
Background Protein S is a vitamin K dependent plasma glycoprotein (MW 69,000) which belongs to a group of coagulation inhibitors consisting itself of proteinases. It circulates in the blood in a free active form and in an inactive form bound to C4b binding protein. Protein S is also released by thrombin stimulation from platelet alpha-granules.
Protein S acts a s a cofactor for activated protein C (pCa) enhancing the cleavage of factors Va and VIIIa, producing anticoagulation by decreasing the conversion of prothrombin to thrombin. It also stimulates the fibrinolytic system. Protein S looses much of its coagulant cofactor activity after cleavage by thrombin, a process which is inhibited by thrombomodulin in the presence of free calcium ions.
Plasma concentration of protein S may be significantly reduced below normal adult levels (2.5 mg/ml) in patients with hereditary deficiency of protein S or suffering a combination of deep vein thrombosis, superficial thrombophlebitis and pulmonary embolism. Substances such as bacterial endotoxins, tumour necrosis factor and immune mediator interleukin 1 also reduce the protein S level in plasma. Oral contraceptives also depress pS activity. A reduction in plasma protein S during pregnancy usually returns to normal after parturition.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of normal goat serum. No foreign proteins added.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
State:
Lyophilized, Delipidated, Heat inactivated, Stable Whole Antiserum without preservatives
Serum
Reconstitution:
Restore by adding 1 ml of sterile distilled water.
Specificity
Specificity:
The defined antibody specificity is restricted to Protein S as tested at the level of sensitivity of Immunoprecipitation techniques and ELISA techniques. A single precipitin line is obtained in Immunoelectrophoresis, bidimensional Electrophoresis and Double Radial Immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified Protein S. The antiserum also reacts with the protein S component in complexes with Protein C or with C4b Binding Protein.
No reaction is obtained with any other plasma protein.
The antiserum does not cross react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Human.
Gene ID 5627

Accessory Products

Proteins and/or Positive Controls

Proteins for Protein S / PROS1 (6 products)

Catalog No. Species Pres. Purity   Source  

Protein S / PROS1

Protein S / PROS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Protein S / PROS1

Protein S / PROS1 Human > 95 % pure by SDS-PAGE.
Single band at 69 kDa on SDS-PAGE gels, and a doublet upon incubation with 2-mercaptoethanol.
Plasma
50 µg / €250.00
  OriGene Technologies GmbH

Protein S / PROS1

Protein S / PROS1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Protein S / PROS1

Protein S / PROS1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Protein S / PROS1

Protein S / PROS1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Protein S / PROS1

Protein S / PROS1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Protein S / PROS1 (2 products)

Catalog No. Species Pres. Purity   Source  

PROS1 293T Cell Transient Overexpression Lysate(Denatured)

PROS1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

Protein S Lysate

Western Blot: Protein S Lysate [NBL1-14812] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PROS1
  Novus Biologicals Inc.
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