Product Description for Protein S / PROS1
Goat anti Human Protein S / PROS1.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.
Properties for Protein S / PROS1
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 1 ml |
| Synonyms | PROS, Vitamin K-dependent protein S |
| Presentation | Serum |
| Reactivity | Hu |
| Applications | ID, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Add. information | Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application | Can be used in precipitating techniques as Immunoelectrophoresis, Single or Double Radial Immunodiffusion, Electroimmunodiffusion, Immunonepholometry as well as solid phase and Neutralization reactions. As catching antibody in ELISA. To prepare an adsorbent for immunoaffinity purification of Protein S. Plasma samples and all assay components must contain EDTA to stabilize the protein. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl Human plasma or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. Single Radial Immunodiffusion: Use 1% antiserum in the gel. Antibody Titre: Precipitin titre 1/32 when tested against in agar-block Immunodiffusion titration. |
| Background | Protein S is a vitamin K dependent plasma glycoprotein (MW 69,000) which belongs to a group of coagulation inhibitors consisting itself of proteinases. It circulates in the blood in a free active form and in an inactive form bound to C4b binding protein. Protein S is also released by thrombin stimulation from platelet alpha-granules. Protein S acts a s a cofactor for activated protein C (pCa) enhancing the cleavage of factors Va and VIIIa, producing anticoagulation by decreasing the conversion of prothrombin to thrombin. It also stimulates the fibrinolytic system. Protein S looses much of its coagulant cofactor activity after cleavage by thrombin, a process which is inhibited by thrombomodulin in the presence of free calcium ions. Plasma concentration of protein S may be significantly reduced below normal adult levels (2.5 mg/ml) in patients with hereditary deficiency of protein S or suffering a combination of deep vein thrombosis, superficial thrombophlebitis and pulmonary embolism. Substances such as bacterial endotoxins, tumour necrosis factor and immune mediator interleukin 1 also reduce the protein S level in plasma. Oral contraceptives also depress pS activity. A reduction in plasma protein S during pregnancy usually returns to normal after parturition. |
| Concentration | Total protein and IgG concentrations in the antiserum are comparable to those of normal goat serum. No foreign proteins added. |
| Storage | Prior to reconstitution store at 2-8°C. |
| Format |
|
| Specificity |
|
| Gene ID | 5627 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Protein S / PROS1 (6 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP302073 |
Protein S / PROS1 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| BA548 |
Protein S / PROS1 |
||||||
|
Human |
> 95 % pure by SDS-PAGE. Single band at 69 kDa on SDS-PAGE gels, and a doublet upon incubation with 2-mercaptoethanol. |
Plasma |
50 µg /
€250.00
|
|||
| OriGene Technologies GmbH | |||||||
| H00005627-P01-10 |
Protein S / PROS1 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005627-P01-25 |
Protein S / PROS1 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005627-Q01-10 |
Protein S / PROS1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005627-Q01-25 |
Protein S / PROS1 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Protein S / PROS1 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00005627-T01 |
PROS1 293T Cell Transient Overexpression Lysate(Denatured) |
||||||
![]() |
|||||||
| Abnova Taiwan Corp. | |||||||
| NBL1-14812 |
Protein S Lysate |
||||||
![]() |
|||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Protein S / PROS1 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1317AP |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317B |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317F |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1317HRP |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1317T |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | TRITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1416R |
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1435C2 |
Goat IgG (H+L chain), adsorbed antibody |
||||||
|
Donkey | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1435C3 |
Goat IgG (H+L chain), adsorbed antibody |
||||||
|
Donkey | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1435C5 |
Goat IgG (H+L chain), adsorbed antibody |
||||||
|
Donkey | Cy5 | F, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
![Western Blot: Protein S Lysate [NBL1-14812] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for PROS1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Protein%20S%20Lysate-Western%20Blot-NBL1-14812-img0001.jpg)
