AR09759PU-N NIT2 (1-276, His-tag)

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50 µg / €400.00
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NIT2

AR09759PU-N

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  • AR09759PU-N

Product Description for NIT2

NIT2.
Presentation: Purified

Properties for NIT2

Product Category Proteins & Growth Factors
Quantity 50 µg
Synonyms Nitrilase homolog 2
Presentation Purified
Molecular weight kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NQR4
Purity > 95 %
Background Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Barqliw K T., et al. (2008) Biochemistry. 47(51):13514-23.
Camarqo A., et al. (2007) Plant Cell. 19(11):3491-503.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human NIT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP
Molecular weight:
(299aa), confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Purity:
>95%
State:
Liquid purified protein
Purified
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