Product Description for Transferrin
Rabbit anti Porcine Transferrin.
Presentation: Biotin
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Presentation: Biotin
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Properties for Transferrin
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 1 ml |
| Synonyms | Beta-1 metal-binding globulin, Serotransferrin, Siderophilin |
| Presentation | Biotin |
| Reactivity | Por |
| Applications | C, Dot, E, ICC/IF, ID, IP, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Add. information | Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate technique for the binding to peroxidase, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. |
| Application | Can be used in Enzyme-Immunocytochemical and Immunohistochemical techniques for the detection of transferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Background | Transferrin is a single polypeptide chain glycoprotein belonging to iron binding family of proteins. It has a molecular weight of 81,5 kDa (porcine). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. |
| Concentration | 10 mg/ml |
| Storage | Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing. Shelf life: one year from despatch. |
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Accessory Products
Recommended Secondary Antibodies for Transferrin (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
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|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
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|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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