CRKL (1-303, His-tag) - AR09797PU-L

Product Description for CRKL

CRKL.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like
Presentation	Purified
Molecular weight	35.9 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P46109
Purity	> 90 %
Background	CRKL is a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substitutete of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and has oncogenic potential.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Arai A., et al. (2001) J Biol Chem. 276(35):33282-90.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human CRKL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE` Molecular weight: (323aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher).
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl Purity: >90% State: Liquid purified protein Purified