Coactosin-like protein (1-142, His-tag) - AR09798PU-N

Product Description for Coactosin-like protein

Coactosin-like protein.
Presentation: Purified

Immunogen	Swiss Prot Num: Q14019
Purity	> 90 %
Background	Coactosin-like protein (COTL1) is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. COTL1 Binds to F-actin in a calcium-independent manner and has no direct effect on actin depolymerization.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Rakonjac M., et al. (2006) Proc Natl Acad Sci U S A. 103(35):13150-5. Provost P., et al. (2001) Biochem J. 359(2):255-63.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human COTL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIKSELK KAGGANYDAQ TE` Molecular weight: (162aa), confirmed by MALDI-TOF
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl Purity: >90% State: Liquid purified protein Purified