Ecotin (eco / eti) (21-162, His-tag) - AR09818PU-L

Product Description for Ecotin (eco / eti)

Ecotin (eco / eti).
Presentation: Purified

Immunogen	Swiss Prot Num: P23827
Purity	> 95 %
Background	Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Lilla JN., et al. (2009) J Biol Chem. 284(20):13792-803. Lund LR., et al. (2006) EMBO J. 25(12):2686-97.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QEDESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVSTMMACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WKAEEKIDNA VVR` Molecular weight: (163aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl, 1mM DTT, 10% glycerol Purity: >95% State: Liquid purified protein Purified