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AF2610 TIMP1 antibody

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Quick Overview

Mouse anti Human TIMP1 147-6D11

AF2610

Product Description for TIMP1

Mouse anti Human TIMP1 147-6D11.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for TIMP1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CLGI, EPA, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Metalloproteinase inhibitor 1, TIMP, TIMP-1, Tissue inhibitor of metalloproteinases
Presentation Purified
Reactivity Hu
Applications P, WB
Clonality Monoclonal
Clone 147-6D11
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01033
GeneID:
7076
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot.
Immunohistochemistry on Paraffin Sections: 2-10 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B).
Concentration 0.5 mg/ml
General Readings
  1. Kodama S, Kishi J, Obata K, Iwata K, Hayakawa T. Monoclonal antibodies to bovine collagenase inhibitor. Coll Relat Res. 1987 Oct;7(5):341-50. PubMed PMID: 3319376.
  2. Okada Y, Takeuchi N, Tomita K, Nakanishi I, Nagase H. Immunolocalization of matrix metalloproteinase 3 (stromelysin) in rheumatoid synovioblasts (B cells): correlation with rheumatoid arthritis. Ann Rheum Dis. 1989 Aug;48(8):645-53. PubMed PMID: 2675782. (Free PMC Article available)
  3. Fukuda, Y. et al. Immunohistochemical study on tissue inhibitors of metalloproteinases in normal and pathological human livers. Gastroenterol. Jpn., 26, 37-41, 1991.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Preservatives:
None
Stabilizers:
0.5% Protease free BSA
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This Monoclonal 147-6D11 antibody specifically reacts with Human Tissue Inhibitor of Metalloproteinases 1 (hTIMP-1). Other species not tested.
Gene ID 7076

Accessory Products

Proteins and/or Positive Controls

Proteins for TIMP1 (16 products)

Catalog No. Species Pres. Purity   Source  

TIMP1
TIMP1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

TIMP1
TIMP1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. 		E. coli
10 µg / €230.00
  OriGene Technologies, Inc.

TIMP1
TIMP1 Human > 90 %
Preparation: .
Purity Detail: >90%, as determined by Coomassie stained SDS-PAGE. 		HEK293 cells
10 µg / €260.00
  OriGene Technologies, Inc.

TIMP1
TIMP1 Human > 95 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

TIMP1
TIMP1 Human > 95 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

TIMP1 (24-207, His-tag)
TIMP1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

TIMP1 (24-207, His-tag)
TIMP1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

TIMP1 (24-207, His-tag)
TIMP1 Human Purified > 90 % by SDS - PAGE
0.25 mg / €1,070.00
  OriGene Technologies GmbH

TIMP1 (24-207, His-tag)
TIMP1 Human Purified > 90 % by SDS - PAGE
50 µg / €320.00
  OriGene Technologies GmbH

TIMP1
TIMP1 Human > 95 % Prepared by affinity chromatography WI-38 cells
  OriGene Technologies GmbH

TIMP1
TIMP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TIMP1
TIMP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TIMP1
TIMP1 Human
  Abnova Taiwan Corp.

TIMP1 (1-207)
Human TIMP1 (Metalloproteinase inhibitor 1) recombinant protein Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunoblotting with anti-MBP (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry E. coli
  Protein Alternatives

TIMP1 (1-207)
Human TIMP1 (Metalloproteinase inhibitor 1) recombinant protein Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunoblotting with anti-MBP (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry E. coli
  Protein Alternatives

TIMP1 (1-207)
Human TIMP1 (Metalloproteinase inhibitor 1) recombinant protein Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunoblotting with anti-MBP (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry E. coli
  Protein Alternatives

Positive controls for TIMP1 (1 products)

Catalog No. Species Pres. Purity   Source  

TIMP1 Lysate
Western Blot: TIMP1 Lysate [NBL1-16924] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TIMP1.
  Novus Biologicals Inc.

Recommended Secondary Antibodies for TIMP1 (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for TIMP1
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