CPSF5 (1-227, His-tag) - AR09875PU-N

Product Description for CPSF5

CPSF5.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.1 mg
Synonyms	CFIM25, CPSF25, Cleavage and polyadenylation specificity factor 25 kDa subunit, Cleavage and polyadenylation specificity factor subunit 5, NUDT21, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit
Presentation	Purified
Molecular weight	28.3 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: O43809
Purity	> 90 %
Background	CPSF5, also called NUDT21, is a member of the Nudix hydrolase family of pyrophosphatases. This protein localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NUDT21 is the smaller subunit of the complex and is present in all heterodimer combinations. NUDT21 plays an important role in pre-mRNA 3' cleavage and polyadenylation processing.
General Readings	Kim S., et al. (2010) Genes Cells. 15(9):1003-13. Shimazu T., et al. (2007) J Biol Chem. 282(7):4470-8.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human NUDT21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN` Molecular weight: (247aa), confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 20% glycerol Purity: >90% State: Liquid purified protein Purified