MMP-1 (active) - AF9110 | acris-antibodies.com

Product Description for MMP-1

Mouse anti Human MMP-1 195-1H6.
Properties: (active)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for MMP-1

Product Category	Primary Antibodies
Quantity	0.1 mg
Synonyms	CLG, Fibroblast collagenase, Interstitial collagenase, MMP1, Matrix metalloproteinase-1
Presentation	Purified
Reactivity	Hu
Applications	P, WB
Clonality	Monoclonal
Clone	195-1H6
Host	Mouse
Isotype	IgG2b
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P03956 Immunogen: A peptide (FVLTEGN, R81-87) of Human Interstitial Collagenase (hMMP-1). GeneID: 4312
Property	active
Isotype control	AM03110PU-N, SM12P
Application	Western blotting. Immunohistochemistry on Paraffin Sections: 2 µg/ml. Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background	The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. MMP1, also known as interstitial collagenase, is the only enzyme that is able to initiate the breakdown of the interstitial collagens, types I, II, and III.
Concentration	0.5 mg/ml
General Readings	Zhang, J. et al. A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 1 (interstitial collagenase) using monoclonal antibodies. Cin. Chim. Acta. 219. 1-14, 1993. Birkedal-Hansen, H. et al. Matrix metalloproteinases: a review. Crit. Rev. Oral. Biol. Med., 4. 197-250, 1993.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
Format	Purification: Affinity Chromatography on Protein A Buffer System: 0.1M Sodium Phosphate buffer, pH 7.0 Preservatives: 0.09% Sodium Azide Stabilizers: 0.5% BSA (Protease-free) State: Liquid purified Ig fraction Purified
Species Reactivity	Species reactivity (tested): Human.
Specificity	Specificity: The antibody specifically reacts with active form of hMMP-1, but not with precursor form of hMMP-1
Gene ID	4312

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-1 (17 products)

Catalog No.	Species	Pres.	Purity	Source
TP302460	MMP-1 (transcript variant 1)
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP720322	MMP-1 (transcript variant 1)
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	10 µg / €299.00
					OriGene Technologies, Inc.
TP723319	MMP-1 (transcript variant 1)
	Human		> 90 % Preparation: . Purity Detail: >90% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / €230.00
					OriGene Technologies, Inc.
TP723882	MMP-1 (transcript variant 1)
	Human		> 90 % Preparation: . Purity Detail: >90%, as determined by Coomassie stained SDS-PAGE.	HEK293 cells	10 µg / €260.00
					OriGene Technologies, Inc.
TP760405	MMP-1 (N-term HIS tag, transcript variant 1)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
					OriGene Technologies, Inc.
AR09435PU-N	MMP-1
	Human		> 90 % pure by SDS-PAGE and HPLC analyses.	E. coli
					OriGene Technologies GmbH
AR09435PU-S	MMP-1
	Human		> 90 % pure by SDS-PAGE and HPLC analyses.	E. coli
					OriGene Technologies GmbH
AR51362PU-N	MMP-1 (100-469, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.5 mg / €820.00
					OriGene Technologies GmbH
AR51362PU-S	MMP-1 (100-469, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.1 mg / €320.00
					OriGene Technologies GmbH
BA440	MMP-1 (Pro)
	Human		> 98 % >95% by SDS-PAGE, essentially free of contaminating MMPs or TIMPs.	Chondrocytes
					OriGene Technologies GmbH
H00004312-P01-10	MMP-1
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00004312-P01-25	MMP-1
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00004312-Q01-10	MMP-1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00004312-Q01-25	MMP-1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P4249	MMP-1
	Human
					Abnova Taiwan Corp.
P4919	MMP-1
	Human
					Abnova Taiwan Corp.
P6072	MMP-1
	Human
					Abnova Taiwan Corp.

Positive controls for MMP-1 (2 products)

Catalog No.	Species
H00004312-T01	MMP1 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.
NBL1-13149	MMP1 Lysate

		Novus Biologicals Inc.

Recommended Secondary Antibodies for MMP-1 (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

Click here to see all secondary antibodies for MMP-1

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products

AF9110 MMP-1 (active) antibody

Quick Overview

Product Description for MMP-1

Properties for MMP-1

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-1 (17 products)

Positive controls for MMP-1 (2 products)

Recommended Secondary Antibodies for MMP-1 (11 products)

Service

Choose your store!