Vimentin - BM5050P | acris-antibodies.com

Product Description for Vimentin

Mouse anti Chicken, Human, Rat, Sheep, Potoroo Vimentin V9.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Vimentin

Product Category	Primary Antibodies
Quantity	0.1 mg
Synonyms	VIM
Presentation	Purified
Reactivity	Chk, Hu, Potoroo, Rt, Sh
Applications	C, F, ICC/IF, P, WB
Clonality	Monoclonal
Clone	V9
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P08670 Immunogen: V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens. GeneID: 7431
Isotype control	AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application	Immunoblotting. Flow Cytometry. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions: 1/25–1/200 for Flow Cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
Background	Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Concentration	1.0 mg/ml
General Readings	Osborn M, Debus E, Weber K. Monoclonal antibodies specific for vimentin. Eur J Cell Biol. 1984 May;34(1):137-43. PubMed PMID: 6428888. Tölle HG, Weber K, Osborn M. Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments. Eur J Cell Biol. 1985 Sep;38(2):234-44. PubMed PMID: 2412818. Van Muijen GN, Ruiter DJ, Warnaar SO. Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest. 1987 Oct;57(4):359-69. PubMed PMID: 3669613. Van Muijen GN, Warnaar SO, Ponec M. Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res. 1987 Aug;171(2):331-45. PubMed PMID: 2442018. Corver WE, Koopman LA, van der Aa J, Regensburg M, Fleuren GJ, Cornelisse CJ. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry. 2000 Feb 1;39(2):96-107. PubMed PMID: 10679727. De Visscher G, Plusquin R, Mesure L, Flameng W. Selection of an immunohistochemical panel for cardiovascular research in sheep. Appl Immunohistochem Mol Morphol. 2010 Jul;18(4):382-91. doi: 10.1097/PAI.0b013e3181cd32e7. PubMed PMID: 20090513.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Buffer System: PBS with 0.09% Sodium Azide as preservative State: Liquid purified IgG fraction Purified
Species Reactivity	Species reactivity (tested): Human, Rat, Chicken, Sheep and Potoroo
Specificity	Specificity: The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Gene ID	7431

Accessory Products

Proteins and/or Positive Controls

Proteins for Vimentin (17 products)

Catalog No.	Species	Pres.	Purity	Source
TP301546	Vimentin
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP723475	Vimentin
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	20 µg / €135.00
					OriGene Technologies, Inc.
AR26022PU-N	Vimentin (1-466)
	Human	Aff - Purified	> 95 % (by SDS-PAGE)	E. coli
					OriGene Technologies GmbH
AR31109PU-N	Vimentin
	Human	Purified	> 95 % pure by SDS-PAGE gel and HPLC analyses	E. coli
					OriGene Technologies GmbH
AR31109PU-S	Vimentin
	Human	Purified	> 95 % pure by SDS-PAGE gel and HPLC analyses	E. coli
					OriGene Technologies GmbH
BA1017	Vimentin
	Human	Purified	> 95 % pure determined by SDS Gel Electrophoresis	E. coli	0.25 mg / €580.00
					OriGene Technologies GmbH
BA1017S	Vimentin
	Human	Purified	> 95 % pure determined by SDS Gel Electrophoresis	E. coli	0.1 mg / €390.00
					OriGene Technologies GmbH
PA1377	Vimentin
	Human	Purified	Greater than 95.0% (SDS-gelelctrophoresis)	E. coli
					OriGene Technologies GmbH
PA1377X	Vimentin
	Human	Purified	Greater than 95.0% (SDS-gelelctrophoresis)	E. coli
					OriGene Technologies GmbH
PA1377XC	Vimentin
	Human	Purified	Greater than 95.0% (SDS-gelelctrophoresis)	E. coli
					OriGene Technologies GmbH
H00007431-P01-10	Vimentin
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00007431-P01-25	Vimentin
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00007431-Q01-10	Vimentin
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00007431-Q01-25	Vimentin
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P1231	Vimentin
	Human
					Abnova Taiwan Corp.
PR27081-2	Vimentin
	Human	Purified	> 95 %	E. coli
					Neuromics Antibodies
PR27081-10	Vimentin
	Human	Purified	> 95 %	E. coli
					Neuromics Antibodies

Positive controls for Vimentin (1 products)

Catalog No.	Species
H00007431-T01	VIM 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.

Recommended Secondary Antibodies for Vimentin (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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