Product Description for Catenin alpha-3 / CTNNA3
Mouse anti Human Catenin alpha-3 / CTNNA3 892_24D2S.
Properties: (164-177)
Presentation: Purified
Product is tested for Flow Cytometry, Paraffin Sections, Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Properties: (164-177)
Presentation: Purified
Product is tested for Flow Cytometry, Paraffin Sections, Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Properties for Catenin alpha-3 / CTNNA3
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Alpha Catenin 3, Alpha T-catenin, Cadherin-associated protein |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | C, F, ICC/IF, IP, P, WB |
| Clonality | Monoclonal |
| Clone | 892_24D2S |
| Host | Mouse |
| Isotype | IgG2a |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | 164-177 |
| Isotype control | AM03096PU-N |
| Application | Western blot. Flow Cytometry. Immunocytochemistry on Acetone-Fixed Cells. Immunohistochemistry on Frozen and Paraffin Sections. Immunoprecipitation. Recommended dilutions: 1/25-1/50 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications. |
| Background | Catenins are proteins that connect cadherin/β- catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by coimmunoprecipitation. |
| Concentration | 1.0 mg/ml |
| General Readings |
|
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 29119 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Catenin alpha-3 / CTNNA3 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP313265 |
Catenin alpha-3 / CTNNA3 (transcript variant 1) |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| H00029119-P01-10 |
Catenin alpha-3 / CTNNA3 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00029119-P01-25 |
Catenin alpha-3 / CTNNA3 |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Catenin alpha-3 / CTNNA3 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00029119-T01 |
CTNNA3 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Catenin alpha-3 / CTNNA3 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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