EIF1B (1-113, His-tag) - AR09903PU-N

Product Description for EIF1B

EIF1B.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.1 mg
Synonyms	Eukaryotic translation initiation factor 1b, GC20, Protein translation factor SUI1 homolog GC20
Presentation	Purified
Molecular weight	kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: O60739
Purity	> 95 %
Background	EIF1B is crucial for the scanning process in vitro. During the scanning process, EIF1B is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. This protein enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Pestova TV., et al. (2002) Genes Dev. 16(22):2906-22. Asano K., et al. (2000) Genes Dec. 14(19):2534-46.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human EIF1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF` Molecular weight: (133aa) confirmed by MALDI-TOF
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl Purity: >95% State: Liquid purified protein Purified