BM6027P CD49f / ITGA6 (6B Isoform specific) antibody

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0.1 mg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human CD49f / ITGA6 6B4

Product Description for CD49f / ITGA6

Mouse anti Human CD49f / ITGA6 6B4.
Properties: (6B Isoform specific)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for CD49f / ITGA6

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6
Presentation Purified
Reactivity Hu
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone 6B4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P23229
Immunogen:
6B4 is a mouse monoclonal IgG1, k antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a synthetic peptide corresponding to a 34 amino acid stretch in the cytoplasmic domain of integrin a6B including an appending N-terminal Cysteine coupled to keyhole limpet hemocyanin.
AA Sequence:
CSRYDDSVPRYHAVRIRKEEREIKDEKYIDNLEKK
GeneID:
3655
Property 6B Isoform specific
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry on Frozen Sections.
Recommended Dilutions: 1/50-1/100 for Flow Cytometry, and for Immunohistochemistry with Avidin-Biotinylated Horseradish Peroxidase Complex (ABC) as detection reagent, and 1/100-1/500 for Immunoblotting applications.
Background Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated a and b subunits. More than 18 a and 8 b subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
For integrin subunits a3 and a6, two cytoplasmic variants, A and B, have been identified.
Concentration 1.0 mg/ml
General Readings
  1. Hogervorst F, Admiraal LG, Niessen C, Kuikman I, Janssen H, Daams H, et al. Biochemical characterization and tissue distribution of the A and B variants of the integrin alpha 6 subunit. J Cell Biol. 1993 Apr;121(1):179-91. PubMed PMID: 7681434. (Free PMC Article available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
It recognizes specifically the cytoplasmic domain of integrin subunit alpha-6B.
6B4 stains the basement membrane zone of ducts and acini of salivary gland, kidney tubules, epithelium of colonic crypts and weakly stains sweat glands and sebaceous glands.
A broad species reactivity is expected because of the conserved nature of the epitope.
Species:
Human.
Gene ID 3655

Accessory Products

Proteins and/or Positive Controls

Proteins for CD49f / ITGA6 (4 products)

Catalog No. Species Pres. Purity   Source  

CD49f / ITGA6 (transcript variant 2)

CD49f / ITGA6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

CD49f / ITGA6

CD49f / ITGA6 Human
  Abnova Taiwan Corp.

CD49f / ITGA6

CD49f / ITGA6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD49f / ITGA6

CD49f / ITGA6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for CD49f / ITGA6 (3 products)

Catalog No. Species Pres. Purity   Source  

Integrin alpha 6 Lysate

Western Blot: Integrin alpha 6 Lysate [NBL1-12066] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for ITGA6
  Novus Biologicals Inc.

ITGA6 293T Cell Transient Overexpression Lysate(Denatured)

ITGA6 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

ITGA6 overexpression lysate

ITGA6 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.
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