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BM6038P Reticulon 1 / RTN1 (Isoform RTN1-A) antibody

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0.1 mg / €370.00
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Quick Overview

Mouse anti Hamster, Human, Mouse, Rat Reticulon 1 / RTN1 MON162

BM6038P

Product Description for Reticulon 1 / RTN1

Mouse anti Hamster, Human, Mouse, Rat Reticulon 1 / RTN1 MON162.
Properties: (Isoform RTN1-A)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Reticulon 1 / RTN1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1
Presentation Purified
Reactivity Hst, Hu, Ms, Rt
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone MON162
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16799
Immunogen:
Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (β-GAL-NSP-A 6-776).
GeneID:
6252
Property Isoform RTN1-A
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Suitable for Immunocytochemistry, Immunohistochemistry on frozen and paraffin embedded tissue, and Immunoblotting.
Recommended dilutions:
1/100-1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting applications.
Background Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non- SCLC (cell cultures). The epitope of MON-162 is located between amino acid residues 338-422 of Reticulon-1A.
Concentration 1.0 mg/ml
General Readings
  1. van de Velde HJ, Roebroek AJ, van Leeuwen FW, Van de Ven WJ. Molecular analysis of expression in rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum. Brain Res Mol Brain Res. 1994 Apr;23(1-2):81-92. PubMed PMID: 7518032.
  2. van de Velde HJ, Roebroek AJ, Senden NH, Ramaekers FC, Van de Ven WJ. NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum. J Cell Sci. 1994 Sep;107 ( Pt 9):2403-16. PubMed PMID: 7844160.
  3. van de Velde HJ, Senden NH, Roskams TA, Broers JL, Ramaekers FC, Roebroek AJ, et al. NSP-encoded reticulons are neuroendocrine markers of a novel category in human lung cancer diagnosis. Cancer Res. 1994 Sep 1;54(17):4769-76. PubMed PMID: 8062278.
  4. Senden NH, Timmer ED, de Bruïne A, Wagenaar SS, Van de Velde HJ, Roebroek AJ, et al. A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers. J Pathol. 1997 May;182(1):13-21. PubMed PMID: 9227337.
  5. Hens J, Nuydens R, Geerts H, Senden NH, Van de Ven WJ, Roebroek AJ, et al. Neuronal differentiation is accompanied by NSP-C expression. Cell Tissue Res. 1998 May;292(2):229-37. PubMed PMID: 9560466.
Storage Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS with 0.09% sodium azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
MON-162 exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues.
Species:
Human, mouse, hamster and rat.
Gene ID 6252

Accessory Products

Proteins and/or Positive Controls

Proteins for Reticulon 1 / RTN1 (4 products)

Catalog No. Species Pres. Purity   Source  

Reticulon 1 / RTN1 (transcript variant 1)
Reticulon 1 / RTN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Reticulon 1 / RTN1 (transcript variant 3)
Reticulon 1 / RTN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Reticulon 1 / RTN1
Reticulon 1 / RTN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Reticulon 1 / RTN1
Reticulon 1 / RTN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Reticulon 1 / RTN1 (1 products)

Catalog No. Species Pres. Purity   Source  

RTN1 overexpression lysate
RTN1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

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Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
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