EGFR / ERBB1 pTyr1069 (incl. pos. control) - AM00034PU-N

Product Description for EGFR / ERBB1

Mouse anti Human, Mouse EGFR / ERBB1 11C2.
Properties: pTyr1069 (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for EGFR / ERBB1

Product Category	Primary Antibodies
Quantity	0.1 mg
Synonyms	EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Presentation	Purified
Reactivity	Hu, Ms
Applications	E, IP, WB
Clonality	Monoclonal
Clone	11C2
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P00533 Immunogen: Phosphopeptide conjugated to KLH. Epitope: Phosphotyrosine pY1069 (L Q R pY S S D). GeneID: 1956
Property	pTyr1069
Control peptide	AM00034CP-N
Isotype control	AM03095PU-N, SM10P (for use in human samples)
Add. information	Mol. weight: 180 kDa.
Application	ELISA: Use at 0.05 µg/ml Immunoblotting. 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells. Positive Control: Cell lysate from pervanadate-treated HepG2 cells (See Protocols for more details).
Background	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. The phosphorylation status of Tyrosine 1069 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1069 creates a docking site for the c-cbl ubiquitination ligase.
Concentration	0.1 mg/ml
Protocols	Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cells Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min with pervanadate. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Storage	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography. Buffer System: PBS, 0.09% Sodium Azide/PEG and Sucrose/50% Glycerol. State: Liquid purified Ig fraction. Purified
Specificity	Specificity: This antibody specifically recognizes EGFR phosphorylated at Tyrosine 1069. Species: Human and Mouse.
Gene ID	1956

Accessory Products

Proteins and/or Positive Controls

Proteins for EGFR / ERBB1 (53 products)

Catalog No.	Species	Pres.	Purity	Source
TP710003	EGFR / ERBB1
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	SF9 cells	20 µg / €411.00
					OriGene Technologies, Inc.
TP314877	EGFR / ERBB1 (transcript variant 3)
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP700043	EGFR / ERBB1
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP700065	EGFR / ERBB1 (C-term MYC/DDK tag, transcript variant 1)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP700080	EGFR / ERBB1 (C-term MYC/DDK tag, transcript variant 1)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP700103	EGFR / ERBB1 (C-term DDK/His tag, transcript variant 3)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP700204	EGFR / ERBB1 (C-term DDK/His tag)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP710011	EGFR / ERBB1 (full length, C-term DDK tag)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	SF9 cells	20 µg / €411.00
					OriGene Technologies, Inc.
TP723071	EGFR / ERBB1 (transcript variant 3)
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	CHO cells	10 µg / €230.00
					OriGene Technologies, Inc.
AR09438PU-N	EGFR / ERBB1
	Human		≥ 95 pure by SDS-PAGE gel and HPLC analyses	CHO cells
					OriGene Technologies GmbH
AR09438PU-S	EGFR / ERBB1
	Human		≥ 95 pure by SDS-PAGE gel and HPLC analyses	CHO cells
					OriGene Technologies GmbH
AR10195PU-L	EGFR / ERBB1 (active protein)
	Human	Purified		Insect cells
					OriGene Technologies GmbH
AR10195PU-N	EGFR / ERBB1 (active protein)
	Human	Purified		Insect cells
					OriGene Technologies GmbH
AR10195PU-S	EGFR / ERBB1 (active protein)
	Human	Purified		Insect cells
					OriGene Technologies GmbH
AR52062PU-N	EGFR / ERBB1 (25-645, hIgG-His tag)
	Human	Purified	> 90 % by SDS - PAGE		50 µg / €600.00
					OriGene Technologies GmbH
AR52062PU-S	EGFR / ERBB1 (25-645, hIgG-His tag)
	Human	Purified	> 90 % by SDS - PAGE		10 µg / €200.00
					OriGene Technologies GmbH
DA3557	EGFR / ERBB1
	Human	Purified	~ 90 % by SDS-PAGE and visualized by silver stain	Insect cells	25 µg / €430.00
					OriGene Technologies GmbH
H00001956-P02	EGFR / ERBB1
	Human	Purified
					Abnova Taiwan Corp.
H00001956-P02-10	EGFR / ERBB1
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00001956-P02-25	EGFR / ERBB1
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00001956-Q01-10	EGFR / ERBB1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00001956-Q01-25	EGFR / ERBB1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00001956-Q03-10	EGFR / ERBB1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue
					Abnova Taiwan Corp.
H00001956-Q03-25	EGFR / ERBB1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue
					Abnova Taiwan Corp.
P1514	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P1542	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P1640	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P2637	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P2641	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P2645	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P2646	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P2647	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P2648	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4676	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4813	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4814	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4815	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4816	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4817	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4818	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4819	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4820	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4821	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P4822	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5697	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5698	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5699	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5700	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5701	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5702	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5737	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
P5971	EGFR / ERBB1
	Human
					Abnova Taiwan Corp.
NBP1-46047	EGFR / ERBB1
	Human	Purified
					Novus Biologicals Inc.

Positive controls for EGFR / ERBB1 (4 products)

Catalog No.	Species
H00001956-T01	EGFR 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.
LY404514	EGFR overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.
LY404515	EGFR overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY417434	EGFR overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Recommended Secondary Antibodies for EGFR / ERBB1 (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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AM00034PU-N EGFR / ERBB1 pTyr1069 (incl. pos. control) antibody

Quick Overview

Product Description for EGFR / ERBB1

Properties for EGFR / ERBB1

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for EGFR / ERBB1 (53 products)

Positive controls for EGFR / ERBB1 (4 products)

Recommended Secondary Antibodies for EGFR / ERBB1 (11 products)

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