Product Description for EGFR / ERBB1
Mouse anti Human, Mouse EGFR / ERBB1 10F4.
Properties: (1140-1160) (incl. pos. control)
Presentation: Biotin
Product is tested for Western blot / Immunoblot.
Properties: (1140-1160) (incl. pos. control)
Presentation: Biotin
Product is tested for Western blot / Immunoblot.
Properties for EGFR / ERBB1
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1 |
| Presentation | Biotin |
| Reactivity | Hu, Ms |
| Applications | WB |
| Clonality | Monoclonal |
| Clone | 10F4 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | 1140-1160 |
| Isotype control | SM10B (for use in human samples) |
| Application | Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. |
| Background | EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling. |
| Concentration | 0.25 mg/ml |
| Storage | Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch. |
| Format |
|
| Specificity |
|
| Gene ID | 1956 |
Accessory Products
Proteins and/or Positive Controls
Proteins for EGFR / ERBB1 (53 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP710003 |
EGFR / ERBB1 |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP314877 |
EGFR / ERBB1 (transcript variant 3) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP700043 |
EGFR / ERBB1 |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP700065 |
EGFR / ERBB1 (C-term MYC/DDK tag, transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP700080 |
EGFR / ERBB1 (C-term MYC/DDK tag, transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP700103 |
EGFR / ERBB1 (C-term DDK/His tag, transcript variant 3) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP700204 |
EGFR / ERBB1 (C-term DDK/His tag) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP710011 |
EGFR / ERBB1 (full length, C-term DDK tag) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723071 |
EGFR / ERBB1 (transcript variant 3) |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
CHO cells |
10 µg /
€230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR09438PU-N |
EGFR / ERBB1 |
||||||
|
Human | ≥ 95 pure by SDS-PAGE gel and HPLC analyses | CHO cells | ||||
| OriGene Technologies GmbH | |||||||
| AR09438PU-S |
EGFR / ERBB1 |
||||||
|
|
Human | ≥ 95 pure by SDS-PAGE gel and HPLC analyses | CHO cells | ||||
| OriGene Technologies GmbH | |||||||
| AR10195PU-L |
EGFR / ERBB1 (active protein) |
||||||
|
|
Human | Purified | Insect cells | ||||
| OriGene Technologies GmbH | |||||||
| AR10195PU-N |
EGFR / ERBB1 (active protein) |
||||||
|
|
Human | Purified | Insect cells | ||||
| OriGene Technologies GmbH | |||||||
| AR10195PU-S |
EGFR / ERBB1 (active protein) |
||||||
|
|
Human | Purified | Insect cells | ||||
| OriGene Technologies GmbH | |||||||
| AR52062PU-N |
EGFR / ERBB1 (25-645, hIgG-His tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE |
50 µg /
€600.00
|
|||
| OriGene Technologies GmbH | |||||||
| AR52062PU-S |
EGFR / ERBB1 (25-645, hIgG-His tag) |
||||||
|
|
Human | Purified | > 90 % by SDS - PAGE |
10 µg /
€200.00
|
|||
| OriGene Technologies GmbH | |||||||
| DA3557 |
EGFR / ERBB1 |
||||||
|
Human | Purified | ~ 90 % by SDS-PAGE and visualized by silver stain | Insect cells |
25 µg /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
| H00001956-P02 |
EGFR / ERBB1 |
||||||
|
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00001956-P02-10 |
EGFR / ERBB1 |
||||||
|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001956-P02-25 |
EGFR / ERBB1 |
||||||
|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001956-Q01-10 |
EGFR / ERBB1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001956-Q01-25 |
EGFR / ERBB1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001956-Q03-10 |
EGFR / ERBB1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue | |||||
| Abnova Taiwan Corp. | |||||||
| H00001956-Q03-25 |
EGFR / ERBB1 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue | |||||
| Abnova Taiwan Corp. | |||||||
| P1514 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P1542 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P1640 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2637 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2641 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2645 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2646 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2647 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P2648 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4676 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4813 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4814 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4815 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4816 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4817 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4818 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4819 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4820 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4821 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4822 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5697 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5698 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5699 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5700 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5701 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5702 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5737 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5971 |
EGFR / ERBB1 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| NBP1-46047 |
EGFR / ERBB1 |
||||||
![Western Blot: EGFR Protein [NBP1-46047] - Lane 1 – MW markers; Lane 2 – EGF R - FcB Chimera; Lane 3 – EGF R - FcB Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4 – EGF R - FcB Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Deep Purple™. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/EGFR%20Protein-Western%20Blot-NBP1-46047-img0001.jpg)
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
Positive controls for EGFR / ERBB1 (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00001956-T01 |
EGFR 293T Cell Transient Overexpression Lysate(Denatured) |
||||||
|
|||||||
| Abnova Taiwan Corp. | |||||||
| LY404514 |
EGFR overexpression lysate |
||||||
|
0.1 mg /
€495.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| LY404515 |
EGFR overexpression lysate |
||||||
|
0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| LY417434 |
EGFR overexpression lysate |
||||||
|
0.1 mg /
€495.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for EGFR / ERBB1 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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