Product Description for Phosphoserine
Mouse anti Canine, Human, Mouse, Rat Phosphoserine 4A3.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.
Properties for Phosphoserine
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Presentation | Purified |
| Reactivity | Can, Hu, Ms, Rt |
| Applications | E, IP, WB |
| Clonality | Monoclonal |
| Clone | 4A3 |
| Host | Mouse |
| Isotype | IgM |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | SM13P |
| Add. information | This product contains a Positive Control (for details see "Protocols"). |
| Application | ELISA: Use at 0.05 µg/ml. Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells. Western Blot (Immunoblotting): 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILK OR CASEIN FOR BLOCKING! |
| Background | Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases. |
| Protocols | Positive Control: pSer / pThr Molecular Weight Marker Formulation The pSer/pThr molecular weight marker contains rabbit muscle phosphoproteins isolated by Fe+/IDA-affinity chromatography. Proteins are lyophilized from PBS/NaF/PEG/SUCROSE/Bromophenolblue and Na-azide. After reconstitution the solution contains 0.09% Na-azide. Stability Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C. Application The pSer/pThr molecular weight marker is recommendede for immunoblot applications. Use 20 µl molecular meight marker per lane. Note: Use BSA based blot incubation buffers. Milk, Casein and Blotto might interfere with antibody - antigen interaction. Storage Aliquote and store frozen. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| General Readings |
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| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 1 week. Shelf life: one year from despatch. |
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Accessory Products
Recommended Secondary Antibodies for Phosphoserine (10 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1349AP |
Mouse IgA (H+L chain) antibody |
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|
Goat | AP | |||||
| OriGene Technologies GmbH | |||||||
| R1349B |
Mouse IgA (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| OriGene Technologies GmbH | |||||||
| R1349F |
Mouse IgA (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| OriGene Technologies GmbH | |||||||
| R1349HRP |
Mouse IgA (+IgG +IgM) antibody |
||||||
|
|
Goat | HRP | |||||
| OriGene Technologies GmbH | |||||||
| R1349T |
Mouse IgA (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| OriGene Technologies GmbH | |||||||
| R1349TR |
Mouse IgA (H+L chain) antibody |
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|
|
Goat | Texas Red | |||||
| OriGene Technologies GmbH | |||||||
| SP1018AP |
Mouse IgM (Mu chain specific), adsorbed antibody |
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|
|
Goat | AP | Ms | ||||
| OriGene Technologies GmbH | |||||||
| SP1018F |
Mouse IgM (Mu chain specific), adsorbed antibody |
||||||
|
|
Goat | FITC | Ms | ||||
| OriGene Technologies GmbH | |||||||
| SP1018HRP |
Mouse IgM (Mu chain specific), adsorbed antibody |
||||||
|
|
Goat | HRP | Ms | C, E, P | |||
| OriGene Technologies GmbH | |||||||
| SP1018R |
Mouse IgM (Mu chain specific), adsorbed antibody |
||||||
|
|
Goat | PE | Ms | ||||
| OriGene Technologies GmbH | |||||||
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