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AM00157PU-N VASP pSer239 (incl. pos. control) antibody

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0.1 mg / €550.00
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Quick Overview

Mouse anti Human, Mouse VASP 22E11

AM00157PU-N

Product Description for VASP

Mouse anti Human, Mouse VASP 22E11.
Properties: pSer239 (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for VASP

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Vasodilator-stimulated phosphoprotein
Presentation Purified
Reactivity Hu, Ms
Applications E, F, WB
Clonality Monoclonal
Clone 22E11
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P50552
Immunogen:
Synthetic phosphopeptide conjugated to KLH.
GeneID:
7408
Property pSer239
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use at 0.05 µg/ml.
Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Flow Cytometry.
Included Positive Control: Cell lysate from Forskolin-treated HepG2 cells (See Protocols for more details).
Background VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.
Protocols Positive Control Cell Lysate:
HepG2 Forskolin treated

Format: Lyophilized cell lysate from HepG2 cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.

Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Shelf life: one year from despatch.
Format
Purification:
Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Buffer System:
2 x PBS/0.09% Na-azide/PEG and Sucrose.
State:
Lyophilized purified IgG fraction.
Purified
Reconstitution:
Restore with 1 ml H2O (15 min, RT).
Specificity
Specificity:
This antibody recognizes VASP only, when Ser239 is phosphorylated, a site preferred by cGMP-dependent protein kinase (PKG) but also used by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-22E11 is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.
Species:
Human and Mouse.
Gene ID 7408

Accessory Products

Proteins and/or Positive Controls

Proteins for VASP (7 products)

Catalog No. Species Pres. Purity   Source  

VASP
VASP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

VASP (1-343, His-tag)
Recombinant human VASP, 1-343aa, His-tagged Human Purified > 85 % E. coli
0.25 mg / €1,070.00
  OriGene Technologies GmbH

VASP (1-343, His-tag)
Recombinant human VASP, 1-343aa, His-tagged Human Purified > 85 % E. coli
50 µg / €400.00
  OriGene Technologies GmbH

VASP
VASP Human Purified
  Abnova Taiwan Corp.

VASP
VASP Human Purified
  Abnova Taiwan Corp.

VASP
VASP Human Purified
  Novus Biologicals Inc.

VASP
VASP Human Purified
  Novus Biologicals Inc.

Positive controls for VASP (2 products)

Catalog No. Species Pres. Purity   Source  

VASP 293T Cell Transient Overexpression Lysate(Denatured)
VASP 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

VASP Lysate
Western Blot: VASP Lysate [NBL1-17699] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for VASP
  Novus Biologicals Inc.

Recommended Secondary Antibodies for VASP (11 products)

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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
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Mouse IgG (H+L chain), adsorbed antibody
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Click here to see all secondary antibodies for VASP
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